Description

Biotin-Phenylalanyl-(S-Benzyl)Cysteinyl-Diazomethane CAS NO 153512-31-3 is a specialized, high-purity synthetic peptide derivative designed for advanced biochemical research and development. This compound is a critical reagent in the field of chemical biology, primarily utilized for the site-specific labeling and cross-linking of proteins. It is an essential tool for researchers and manufacturers in the pharmaceutical and biotechnology sectors who require precise molecular probes for studying protein function, interaction, and structure. Its unique structure, incorporating biotin, a diazomethane group, and protected amino acids, makes it invaluable for affinity-based assays and targeted drug discovery platforms.

Application

• Proteomics Research: As a key reagent for the biotinylation and subsequent isolation of specific proteins or protein complexes in affinity purification workflows (e.g., pull-down assays).
• Chemical Biology Tool Development: For the creation of novel molecular probes that enable the study of enzyme mechanisms, protein-protein interactions, and post-translational modifications.
• Pharmaceutical R&D: In the discovery and development of targeted therapeutics, particularly for creating antibody-drug conjugates (ADCs) or other bioconjugates where site-specific labeling is crucial.
• Diagnostic Assay Development: Used in the formulation of sensitive immunoassays and biosensors that rely on high-affinity biotin-streptavidin technology for signal amplification.
• Material Science & Surface Chemistry: For functionalizing surfaces (e.g., chips, beads) with bioactive molecules to create platforms for high-throughput screening or diagnostic devices.

Basic Information

Product Name	Biotin-Phenylalanyl-(S-Benzyl)Cysteinyl-Diazomethane
CAS No.	153512-31-3
Molecular Formula	C₃₀H₃₈N₆O₅S₂
Molecular Weight	626.78 g/mol
Synonyms	Biotin-Phe-(S-Bzl)Cys-CHN₂; Biotin-L-Phenylalanyl-L-cysteine(S-benzyl) methyl ester diazomethane; N-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[(2S)-1-[[(2S)-3-(benzylsulfanyl)-1-diazo-1-oxopropan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]pentanediamide; Biotinylated Peptide Diazomethane Derivative; Affinity Labeling Reagent; Protein Cross-linking Agent
EINECS	Contact for details

Quality Control

Our Biotin-Phenylalanyl-(S-Benzyl)Cysteinyl-Diazomethane is manufactured under strict quality control protocols to ensure the highest standards of purity and consistency required for research and development. Each batch is characterized using advanced analytical techniques including HPLC, mass spectrometry, and NMR to confirm identity and purity. Certificates of Analysis (COA) detailing lot-specific results are provided with every shipment. We adhere to cGMP guidelines where applicable to support our clients in regulated development pathways.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For long-term stability, store under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.