Description

Hedgehog Protein, Drosophila CAS NO 149291-21-4 is a highly purified recombinant protein derived from the Drosophila melanogaster (fruit fly) Hedgehog signaling molecule. This protein is a critical reagent for fundamental research in developmental biology, stem cell regulation, and tissue patterning. It is essential for scientists and R&D teams in academic, biotechnology, and pharmaceutical sectors who require a biologically active, well-characterized ligand to study the highly conserved Hedgehog signaling pathway in vitro and in vivo.

Application

• Developmental Biology Research: Used to study cell fate determination, embryonic patterning, and organogenesis in model systems.
• Stem Cell & Regenerative Medicine: Applied in protocols to direct the differentiation of stem cells for potential therapeutic applications.
• Cancer Research: Employed to investigate the role of aberrant Hedgehog signaling in tumorigenesis and cancer cell proliferation.
• High-Throughput Screening (HTS): Serves as a key component in assays designed to identify novel agonists or antagonists of the Hedgehog pathway for drug discovery.
• Biochemical Assay Development: Used to establish binding kinetics, receptor activation studies, and downstream signaling readouts.
• Cell Culture & Tissue Engineering: Added to media to maintain or induce specific cellular phenotypes in specialized culture systems.

Basic Information

Product Name	Hedgehog Protein, Drosophila
CAS No.	149291-21-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Drosophila Hedgehog Protein; Dhh Protein (Drosophila); Recombinant Drosophila Hedgehog; Hh Protein (Drosophila melanogaster); Hedgehog Signaling Protein; Morphogen Hedgehog; Hedgehog N-terminal fragment; Hedgehog signaling molecule
EINECS	Contact for details

Quality Control

Our Hedgehog Protein, Drosophila is manufactured under stringent conditions to ensure high biological activity and batch-to-batch consistency. Each lot is subjected to rigorous analytical testing, including purity assessment, endotoxin level determination, and functional activity verification via cell-based bioassays. A comprehensive Certificate of Analysis (COA) detailing identity, purity, potency, and safety specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. Aliquot upon receipt for single-use applications to maintain optimal activity.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band at expected molecular weight
Purity (HPLC)	≥ 95%
Biological Activity (Cell-based assay)	Reported on COA
Endotoxin Level (LAL)	< 1.0 EU/µg
Protein Content (BCA/A280)	Reported on COA
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.