Description

Leupeptin Trifluoroacetate Salt is a potent, cell-permeable serine and cysteine protease inhibitor. This compound is critical for researchers and manufacturers requiring precise control over proteolytic activity in biological systems. It is primarily utilized in life science research, pharmaceutical development, and bioprocessing applications where the inhibition of enzymes like trypsin, plasmin, and cathepsin B is essential.

Application

• Biomedical Research: Used as a key reagent in cell culture and biochemical assays to study apoptosis, protein degradation pathways, and enzyme kinetics.
• Pharmaceutical Development: Employed in the purification and stabilization of therapeutic proteins and peptides by preventing proteolytic degradation during manufacturing processes.
• Diagnostic Reagent Production: Incorporated into diagnostic kits and buffers to inhibit unwanted protease activity, ensuring assay stability and accuracy.
• Enzymology Studies: Serves as a standard inhibitor for characterizing the specificity and function of serine and cysteine proteases in vitro.
• Bioprocessing: Applied in downstream processing to protect valuable biological products from protease contamination, improving yield and purity.

Basic Information

Product Name	Leupeptin Trifluoroacetate Salt
CAS No.	147385-61-3
Molecular Formula	C₂₀H₃₈N₆O₄ • xC₂HF₃O₂
Molecular Weight	~463.5 (for free base, excluding TFA counterion)
Synonyms	Leupeptin Hemisulfate (alternative salt); Acetyl-Leu-Leu-Arg-al; N-Acetyl-L-leucyl-L-leucyl-L-argininal; Protease Inhibitor, Leupeptin; NSC 332039; Antibiotic, Leupeptin; Leupeptins
EINECS	Contact for details

Quality Control

Our Leupeptin Trifluoroacetate Salt is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and industrial use. Certificates of Analysis (COA) detailing purity, potency, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residue on Ignition	≤ 1.0%
Solubility	Soluble in water, DMSO, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.