Description

Cyclothialidine is a potent and specific inhibitor of bacterial DNA gyrase, a critical enzyme for DNA replication. This targeted mechanism makes it a valuable tool compound for microbiological research and a promising scaffold for the development of novel antibacterial agents. It is primarily utilized by pharmaceutical R&D departments, academic research institutions, and biotechnology companies focused on infectious disease and antibiotic discovery.

Application

• Antibacterial Research & Development: Serves as a key reference standard and lead compound in the discovery of new classes of antibiotics targeting DNA gyrase.
• Mechanistic Studies: Used in biochemical and microbiological assays to study the function and inhibition of bacterial DNA gyrase and topoisomerase IV.
• Structure-Activity Relationship (SAR) Analysis: Employed as a core structure for medicinal chemistry programs to optimize potency and pharmacokinetic properties.
• Microbiological Tool: Applied in laboratory settings to selectively inhibit Gram-positive bacterial growth for research purposes.
• High-Throughput Screening (HTS): Utilized as a control compound in screening campaigns to identify novel gyrase inhibitors.

Basic Information

Product Name	Cyclothialidine
CAS No.	147214-63-9
Molecular Formula	C₂₄H₂₈N₂O₇S
Molecular Weight	488.55 g/mol
Synonyms	Cyclothialidine; Ro 09-1437; L-695,256; (1R,5S,6S)-6-[(1R)-1-Hydroxyethyl]-1-methyl-4-[[(4-methyl-1,3-thiazol-2-yl)carbonyl]amino]-7-oxo-2-thia-4,8-diazabicyclo[3.3.0]oct-3-ene-3-carboxylic acid 5,5-dioxide; DNA Gyrase Inhibitor Ro 09-1437
EINECS	Contact for details

Quality Control

Our Cyclothialidine is produced and handled under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods for structural confirmation, to ensure it meets the high standards required for research applications. A detailed Certificate of Analysis (COA) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. For short-term use, the material may be stored at 2-8°C. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residue on Ignition	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.