Description

Y-27632 Dihydrochloride is a potent, cell-permeable, and selective inhibitor of Rho-associated coiled-coil containing protein kinase (ROCK). This compound is a critical research tool for investigating cellular processes involving the Rho/ROCK signaling pathway, such as cytoskeletal reorganization, cell adhesion, and migration. It is essential for researchers and developers in the fields of cell biology, neuroscience, and drug discovery, particularly for studies involving stem cell differentiation, neuronal regeneration, and vascular biology.

Application

• Cell Biology Research: Used to inhibit ROCK activity in studies of actin cytoskeleton dynamics, cell motility, and cytokinesis.
• Neuroscience Studies: Facilitates research into axonal growth, neuronal survival, and regeneration following injury by modulating Rho/ROCK signaling.
• Stem Cell Culture: Enhances the survival and cloning efficiency of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in single-cell passaging protocols.
• Cardiovascular Research: Investigated for its effects on vascular smooth muscle cell contraction, endothelial function, and blood pressure regulation.
• Drug Discovery Screening: Serves as a reference standard and pharmacological tool in high-throughput screening assays targeting the ROCK pathway for therapeutic development.
• Ophthalmology Research: Applied in studies related to intraocular pressure and glaucoma, due to ROCK's role in trabecular meshwork cell contractility.

Basic Information

Product Name	Y-27632 Dihydrochloride
CAS No.	146986-50-7
Molecular Formula	C₁₄H₂₁N₅O•2HCl
Molecular Weight	338.27 g/mol (free base: 267.35)
Synonyms	(R)-(+)-trans-N-(4-Pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide dihydrochloride; Y27632; Y 27632; Y-27632 2HCl; ROCK Inhibitor Y-27632; Fasudil hydrochloride analog; Rho kinase inhibitor Y-27632
EINECS	Contact for details

Quality Control

Our Y-27632 Dihydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and residual solvent analysis. Certificates of Analysis (COA) detailing batch-specific results are provided to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.