Description

2-Methyl-D3-Citric Acid is a deuterium-labeled isotopologue of 2-methylcitric acid, a key intermediate in the metabolism of propionic acid and branched-chain amino acids. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring analytical accuracy and reliability. It is primarily required by research institutions, pharmaceutical companies, and diagnostic laboratories engaged in metabolic disorder research, drug development, and clinical diagnostics.

Application

• Internal Standard for LC-MS/MS Analysis: Critical for the accurate quantification of 2-methylcitric acid in biological samples (plasma, urine, tissues) for diagnosing propionic and methylmalonic acidemias.
• Metabolic Pathway Tracing: Used in stable isotope tracer studies to investigate the propionate and branched-chain amino acid catabolic pathways.
• Pharmaceutical Research & Development: Employed in pharmacokinetic (PK) and pharmacodynamic (PD) studies for drugs targeting metabolic enzymes.
• Clinical Diagnostics: A vital component in the manufacture of in-vitro diagnostic (IVD) kits and calibrators for newborn screening and metabolic testing.
• Biochemical Research: Serves as a reference compound for enzyme activity assays (e.g., 2-methylcitrate dehydratase) and metabolic profiling.
• Quality Control & Method Validation: Used to validate and ensure the precision of analytical methods in compliance with GLP and ISO 17025 standards.

Basic Information

Item	Details
Product Name	2-Methyl-D3-Citric Acid
CAS No.	146764-58-1
Molecular Formula	C7H7D3O7
Molecular Weight	209.17 g/mol
Synonyms	(2R,3S)-2-Methyl-3-deuterio-2,3-dideuteriocitric Acid; 2-Methylcitric Acid-d3; (2R,3S)-2-Methylcitric Acid-d3; 2-Methyl-3-hydroxy-1,2,3,4-butanetetracarboxylic Acid-d3; 2-Methyl-3-hydroxybutane-1,2,3,4-tetracarboxylic Acid-d3; 3-Carboxy-2-methyl-3-hydroxypentanedioic Acid-d3; 3-Hydroxy-3-carboxy-2-methylpentanedioic Acid-d3
EINECS	Contact for details

Quality Control

Our 2-Methyl-D3-Citric Acid is manufactured under controlled conditions to ensure high isotopic and chemical purity, meeting the stringent requirements of analytical and research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (D, atom %), chemical purity, and impurity profile as determined by advanced techniques like NMR and HPLC-MS. We adhere to a rigorous quality management system, and specifications can be tailored to meet specific research or diagnostic kit manufacturing standards.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a cool, dry, and well-ventilated place. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed after opening to prevent absorption of atmospheric moisture, which could affect purity and stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (D, atom %)	≥ 98.0%
Chemical Purity (by titration)	≥ 97.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.