Description

trans-3'-Hydroxycotinine-n-B-D-Glucuronide is a major, stable metabolite of nicotine, formed via the glucuronidation of trans-3'-hydroxycotinine. This compound is of critical importance as a reference standard and biomarker in clinical and forensic toxicology for the accurate quantification of nicotine exposure and metabolism. It is primarily required by analytical laboratories, contract research organizations (CROs), and pharmaceutical companies engaged in smoking cessation research, pharmacokinetic studies, and compliance monitoring programs.

Application

• Biomarker Standard for Clinical Toxicology: Used as a primary calibrator and quality control material in LC-MS/MS and HPLC assays to measure nicotine metabolite levels in biological matrices like urine, plasma, and saliva.
• Pharmacokinetic & Metabolism Research: Essential for studying the metabolic pathways, clearance rates, and half-life of nicotine and its derivatives in smoking cessation and nicotine replacement therapy studies.
• Forensic Analysis & Workplace Testing: Serves as a definitive reference compound in forensic laboratories for confirming tobacco use in legal, occupational health, and insurance-related testing.
• Diagnostic Kit Development: Employed in the manufacturing and validation of commercial immunoassay kits and point-of-care testing devices designed to detect tobacco exposure.
• Regulatory Compliance & Method Validation: Critical for laboratories seeking to validate analytical methods according to FDA, EMA, or other regulatory guidelines for bioanalytical method validation.
• Academic Research: Used in university and institutional research exploring the long-term health effects of smoking, nicotine addiction mechanisms, and population-level exposure assessments.

Basic Information

Product Name	trans-3'-Hydroxycotinine-n-B-D-Glucuronide
CAS No.	146275-18-5
Molecular Formula	C₁₆H₂₂N₂O₈
Molecular Weight	370.36 g/mol
Synonyms	trans-3'-Hydroxycotinine Glucuronide; (trans)-3'-Hydroxycotinine β-D-Glucuronide; 3HC-Gluc; 3'-Hydroxycotinine O-β-D-Glucuronide; Cotinine-3-glucuronide; Nicotine Metabolite Reference Standard; 1-[(3R)-1-Methyl-5-(3-pyridinyl)-2-pyrrolidinonyl]-β-D-Glucopyranosiduronic Acid
EINECS	Contact for details

Quality Control

Every batch of trans-3'-Hydroxycotinine-n-B-D-Glucuronide is manufactured and analyzed under strict quality management systems. Our products undergo rigorous identity, purity, and stability testing using advanced analytical techniques including HPLC, LC-MS, and NMR to ensure compliance with the high standards required for reference materials. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This compound is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For extended storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.