Description

trans-3'-Hydroxy Cotinine Benzoate is a high-purity benzoate derivative of the nicotine metabolite, trans-3'-hydroxycotinine. This compound is a critical reference standard and intermediate in analytical chemistry and pharmaceutical research, offering precise quantification and structural validation. It is essential for researchers and manufacturers in the pharmaceutical, diagnostic, and forensic toxicology sectors who require reliable, well-characterized materials for method development and quality control.

Application

• Pharmaceutical Reference Standard: Primary use as a certified reference material (CRM) for the quantification of nicotine metabolites in pharmacokinetic and bioanalytical studies.
• Diagnostic Reagent Manufacturing: Key intermediate in the synthesis of reagents and conjugates for immunoassays and diagnostic kits targeting cotinine levels.
• Forensic & Clinical Toxicology: Used to calibrate equipment (LC-MS/MS, GC-MS) for accurate detection and confirmation of tobacco use biomarkers in biological samples.
• Metabolite Research: Serves as a pure standard for investigating the metabolic pathways and clearance rates of nicotine and related alkaloids.
• Method Development & Validation: Critical for developing, optimizing, and validating analytical methods in compliance with GLP and ISO 17025 standards.
• Chemical Synthesis Intermediate: Used as a protected or derivatized form of hydroxycotinine for further synthetic modifications in medicinal chemistry.

Basic Information

Product Name	trans-3'-Hydroxy Cotinine Benzoate
CAS No.	146275-16-3
Molecular Formula	C17H18N2O3
Molecular Weight	298.34 g/mol
Synonyms	trans-3'-Hydroxycotinine Benzoate; (5S)-1-Methyl-5-(3-pyridyl)-2-pyrrolidinone 3'-Benzoate; 3'-Benzoyloxycotinine; Cotinine, 3'-hydroxy-, benzoate (ester), trans-; 1-Methyl-5-(3-pyridinyl)-2-pyrrolidinone 3'-(benzoate); trans-3'-Benzoyloxycotinine; Hydroxycotinine Benzoate Ester
EINECS	Contact for details

Quality Control

Our trans-3'-Hydroxy Cotinine Benzoate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, IR and NMR for structural confirmation, and residual solvent analysis to ensure compliance with high-purity standards for reference materials. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept under anhydrous conditions. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.