Description

Oncoprotein E2A-Pbx1 CAS NO 146150-85-8 is a high-purity, recombinant fusion protein critical for advanced oncology and molecular biology research. This product is essential for studying the mechanisms of leukemogenesis, specifically pre-B-cell acute lymphoblastic leukemia (ALL), where the E2A-PBX1 chimeric oncogene is a key driver. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and diagnostic developers focused on cancer biology, target validation, and therapeutic discovery.

Application

• Biomarker Research & Validation: Used as a standard or control in assays to detect and quantify the E2A-PBX1 fusion transcript or protein in leukemia studies.
• Drug Discovery & Screening: Serves as a critical target protein in high-throughput screening (HTS) platforms to identify and validate novel small-molecule inhibitors or therapeutic antibodies.
• Mechanistic Studies: Enables fundamental research into the transcriptional dysregulation, signaling pathways, and protein-protein interactions driven by the E2A-PBX1 oncogene.
• Diagnostic Assay Development: Acts as a key reagent in the development and calibration of ELISA, Western Blot, or other immunoassays for clinical and research diagnostics.
• Cell Biology & Functional Genomics: Utilized in cellular transfection and gene expression studies to model leukemogenesis and investigate oncogenic transformation.
• Reference Standard Production: Provides a characterized standard for quality control in the production of related research kits, antibodies, and recombinant proteins.

Basic Information

Product Name	Oncoprotein E2A-Pbx1
CAS No.	146150-85-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	E2A-PBX1 Fusion Protein; t(1;19) Fusion Oncoprotein; Pre-B-cell Leukemia Transcription Factor 1; E2A-PBX1 Chimeric Protein; PBX1/E2A; TCGF3-PBX1; Recombinant E2A-PBX1; E2A-PBX1 Oncogene Product
EINECS	Contact for details

Quality Control

Our Oncoprotein E2A-Pbx1 is manufactured under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes comprehensive analytical testing, including purity assessment by SDS-PAGE and HPLC, identity confirmation by mass spectrometry and Western Blot, and functional activity assays. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to prevent degradation. Store in a dedicated freezer away from volatile substances.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE/Western Blot)	Complies
Purity (SDS-PAGE)	≥ 90%
Purity (HPLC)	≥ 95%
Protein Content (BCA/Bradford)	≥ 90% of stated concentration
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity	Confirmed by functional assay (e.g., DNA-binding, reporter assay)
Solubility	Reconstitutes clearly in specified buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.