Description

Antigen 85B, Mycobacterium Leprae is a purified protein complex derived from the causative agent of leprosy. This antigen is a critical reagent for immunological research, diagnostic development, and vaccine studies targeting *Mycobacterium leprae* infections. It is primarily utilized by research institutions, diagnostic manufacturers, and pharmaceutical companies engaged in infectious disease and immunology research.

Application

• Immunoassay Development: A key component for developing and calibrating ELISA, Western Blot, and other serological tests for leprosy.
• Antibody Production: Used as an immunogen to generate specific polyclonal or monoclonal antibodies for research and diagnostic use.
• Vaccine Research: Serves as a target antigen in studies aimed at evaluating vaccine candidates and understanding protective immune responses against leprosy.
• Basic Immunology Research: Employed to study host-pathogen interactions, T-cell responses, and the immunopathology of leprosy.
• Diagnostic Kit Component: Integrated into commercial point-of-care and laboratory-based diagnostic kits for the detection of *M. leprae*-specific antibodies.
• Quality Control Reference: Acts as a reference standard for the quality control of other diagnostic reagents and assays.

Basic Information

Product Name	Antigen 85B, Mycobacterium Leprae
CAS No.	146045-22-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Mycobacterium leprae Antigen 85B; M. leprae Ag85B; Fibronectin-binding protein Ag85B; Antigen 85 Complex B; Secretory Antigen 85B; 30 kDa Antigen; α-Antigen; MPB59 (Mycobacterium Protein B59); P29 (29-31 kDa Antigen)
EINECS	Contact for details

Quality Control

Our Antigen 85B is produced and purified under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and HPLC, specificity verification via immunoblotting, and functional activity assessment. A comprehensive Certificate of Analysis (COA) detailing purity, protein concentration, endotoxin levels, and biological activity is provided with every shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted aliquots should be stored at -70°C or lower and avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band corresponding to ~30-31 kDa
Purity (HPLC)	≥ 95%
Protein Content (BCA)	≥ 90% of dry weight
Endotoxin Level (LAL)	< 1.0 EU/μg
Biological Activity (ELISA)	Reactive with specific anti-M. leprae antibodies
Solubility	Soluble in aqueous buffers (e.g., PBS)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.