Description

Concanamycin E is a potent and specific macrolide antibiotic belonging to the concanamycin family, identified by CAS NO 144450-35-1. It serves as a highly effective and selective inhibitor of vacuolar-type H+-ATPase (V-ATPase), a critical enzyme complex involved in intracellular pH regulation. This compound is an essential research tool for scientists in cell biology, pharmacology, and biochemistry studying processes like autophagy, receptor-mediated endocytosis, and osteoclast-mediated bone resorption.

Application

• Biomedical Research: As a selective V-ATPase inhibitor to study intracellular pH homeostasis, organelle acidification, and membrane trafficking pathways.
• Cancer Research: Investigation of tumor cell metabolism, drug resistance mechanisms, and the role of V-ATPase in cancer cell survival and metastasis.
• Neuroscience Studies: Research into neurotransmitter storage and release in synaptic vesicles, which relies on V-ATPase activity.
• Microbiology & Immunology: Study of pathogen-host interactions, including the survival of intracellular bacteria and viruses that depend on host cell vesicular acidification.
• Bone Biology: Exploration of osteoclast function and bone resorption processes for potential therapeutic applications in osteoporosis.
• Plant Biology: Research on plant cell vacuolar functions and ion transport mechanisms.
• Pharmacological Probe: Used as a standard or reference compound in high-throughput screening (HTS) assays to discover new V-ATPase modulators.
• Biochemical Assay Development: Essential component in developing and validating in vitro enzymatic assays for V-ATPase activity and inhibition.

Basic Information

Product Name	Concanamycin E
CAS No.	144450-35-1
Molecular Formula	C46H75NO14
Molecular Weight	866.1 g/mol
Synonyms	Concanamycin E; Antibiotic X 4357B; V-ATPase Inhibitor Concanamycin E; Folimycin; (3E,5E,7E,9E,11E,13E,15E)-17-[(2R,3S,4S,5S,6R)-4-(Dimethylamino)-3,5-dihydroxy-6-methyloxan-2-yl]oxy-5-ethyl-4,6,8,10,12,14,16-heptamethyl-17-oxooctadeca-3,5,7,9,11,13,15-heptaen-2-yl 2,4-di-O-methyl-6-deoxy-α-L-mannopyranoside
EINECS	Contact for details

Quality Control

Our Concanamycin E is produced under stringent conditions to ensure the highest standards of purity and biological activity, suitable for sensitive research applications. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and MS to confirm identity and potency. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and reproducibility for your research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep desiccated under an inert atmosphere. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 98% (on dried basis)
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Biological Activity (IC50)	Specified per batch (nM range)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.