Description

4-Trifluoromethyl-7-Hydroxycoumarin Glucuronide Potassium Salt is a high-purity, conjugated metabolite derivative of a prominent coumarin-based fluorophore. This compound is critically important as a key reference standard and enzymatic substrate in advanced biochemical research and analytical method development. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and diagnostic kit manufacturers for studies involving glucuronidation pathways, enzyme activity assays, and fluorescence-based detection.

Application

• Biochemical Research: Acts as a specific fluorescent substrate for studying UDP-glucuronosyltransferase (UGT) enzyme kinetics and inhibition profiles.
• Analytical Reference Standard: Serves as a critical calibrant and internal standard in HPLC, LC-MS, and LC-MS/MS methods for metabolite identification and quantification in drug metabolism and pharmacokinetics (DMPK) studies.
• Diagnostic Development: Used in the formulation of fluorogenic assays and kits for measuring glucuronidase or glucuronosyltransferase activity in clinical and research settings.
• Toxicology & Metabolomics: Employed as a probe to investigate phase II drug metabolism, detoxification pathways, and interspecies differences in glucuronidation.
• High-Throughput Screening (HTS): Utilized in fluorescence-based assays for screening potential drug candidates that modulate UGT enzyme activity.

Basic Information

Product Name	4-Trifluoromethyl-7-Hydroxycoumarin Glucuronide Potassium Salt
CAS No.	143547-78-8
Molecular Formula	C₁₆H₁₃F₃KO₉
Molecular Weight	460.36 g/mol
Synonyms	7-Hydroxy-4-(trifluoromethyl)coumarin glucuronide potassium salt; HFC Glucuronide K⁺ salt; 4-Trifluoromethylumbelliferone glucuronide potassium salt; 4-TFMUG K⁺; Potassium 4-trifluoromethyl-7-β-D-glucopyranuronosyloxy-2H-1-benzopyran-2-one; 7-Hydroxy-4-trifluoromethylcoumarin β-D-glucuronide potassium salt; Fluorescent glucuronide metabolite standard
EINECS	Contact for details

Quality Control

Every batch of our 4-Trifluoromethyl-7-Hydroxycoumarin Glucuronide Potassium Salt is manufactured and tested under a strict quality management system. We provide comprehensive analytical data to ensure identity, purity, and consistency, meeting the stringent requirements for research and diagnostic applications. A detailed Certificate of Analysis (COA) including results from HPLC, NMR, and MS is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	< 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.