Description

Sulfo-n-Succinimidyl-4-(Fluorosulfonyl)Benzoate is a highly reactive, heterobifunctional crosslinking agent designed for advanced bioconjugation and protein modification workflows. Its core value lies in its dual functionality, combining an amine-reactive N-hydroxysuccinimide (NHS) ester with a sulfonyl fluoride group that reacts with phenols and tyrosine residues under mild, aqueous conditions. This compound is essential for researchers and manufacturers in the fields of biopharmaceutical development, proteomics, and diagnostic assay manufacturing, where precise and stable protein labeling is required.

Application

• Antibody-Drug Conjugate (ADC) Development: For site-specific conjugation of cytotoxic payloads to monoclonal antibodies via tyrosine residues.
• Protein Immobilization: Covalent attachment of proteins, enzymes, or antibodies to solid supports (e.g., biosensors, microarrays, chromatographic resins) for assay and purification platforms.
• Fluorescent Labeling of Biomolecules: Conjugation of fluorescent dyes to proteins, peptides, or oligonucleotides for imaging, flow cytometry, and in vitro diagnostics (IVD).
• Peptide and Protein Crosslinking: Studying protein-protein interactions or creating stable protein complexes for structural biology.
• Polymer Functionalization: Introducing bioactive ligands or reporter groups onto polymeric materials for drug delivery systems or tissue engineering scaffolds.
• Diagnostic Reagent Synthesis: Manufacturing key components for immunoassays (ELISA, lateral flow tests) by conjugating detection enzymes or tags to antibodies.

Basic Information

Product Name	Sulfo-n-Succinimidyl-4-(Fluorosulfonyl)Benzoate
CAS No.	142439-47-2
Molecular Formula	C11H7FNO8S2
Molecular Weight	363.30 g/mol
Synonyms	4-Fluorosulfonylbenzoic acid sulfo-NHS ester; Sulfo-NHS-4-(Fluorosulfonyl)benzoate; SFB sulfo-NHS ester; 4-(Fluorosulfonyl)benzoic acid N-hydroxysulfosuccinimide ester; 4-Sulfo-2,3,5,6-tetrafluorophenyl 4-fluorosulfonylbenzoate (related compound); Heterobifunctional crosslinker SFB-NHS; Tyrosine-specific crosslinking agent; SSFB
EINECS	Contact for details

Quality Control

Our Sulfo-n-Succinimidyl-4-(Fluorosulfonyl)Benzoate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) is provided, detailing purity (typically ≥95% by HPLC), identity confirmation, and moisture content. We support GMP-grade production for pharmaceutical applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; partially soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.