Description

Biotinylphenylalanylchloromethane CAS NO 142009-30-1 is a specialized, high-purity biochemical reagent designed for targeted proteomics research and enzyme inhibition studies. This compound is a biotinylated, irreversible serine protease inhibitor, offering a powerful tool for affinity labeling and protein purification. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors for developing novel therapeutics and advanced diagnostic assays.

Application

• Protease Inhibition Studies: Acts as an active-site directed, irreversible inhibitor for characterizing serine proteases.
• Affinity Labeling & Protein Purification: The biotin tag enables efficient streptavidin-based pull-down assays and isolation of target enzymes or their complexes.
• Biochemical Research Reagent: A critical tool in mechanistic enzymology for studying protease function, substrate specificity, and catalytic mechanisms.
• Pharmaceutical Development: Used in early-stage drug discovery for target validation and the development of protease-targeted therapeutic agents.
• Diagnostic Kit Manufacturing: Incorporated into assay systems for the detection and quantification of specific protease activities.
• Chemical Biology Probes: Serves as a foundational building block for creating more complex activity-based probes (ABPs) for proteomic profiling.

Basic Information

Product Name	Biotinylphenylalanylchloromethane
CAS No.	142009-30-1
Molecular Formula	C21H30ClN3O4S
Molecular Weight	455.99 g/mol
Synonyms	Biotin-Phe-CH2Cl; Biotinyl-Phenylalanyl-Chloromethane; (3aS,4S,6aR)-2-[2-[[(2S)-2-[[(Chloromethyl)carbonyl]amino]-3-phenylpropanoyl]amino]ethyl]-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-one; Biotinylated Phenylalanyl Chloromethyl Ketone; Biotin-L-Phe-CH2Cl; Protease Inhibitor (Biotinylated); Biotin-Phe-CMK
EINECS	Contact for details

Quality Control

Every batch of Biotinylphenylalanylchloromethane is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure identity, purity, and performance for critical research applications. A detailed Certificate of Analysis (COA), including HPLC purity, NMR, and mass spectrometry confirmation, is supplied with each shipment to guarantee compliance with your research specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to reach room temperature before opening to prevent condensation and moisture uptake. Store in a dry, well-ventilated area.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.