Description

n-Biotinyl-N'-Maleimido-Ethylenediamine is a high-purity, heterobifunctional crosslinking reagent designed for precise bioconjugation. This compound is critically important for researchers and developers in the life sciences, enabling the stable and site-specific labeling of biomolecules such as proteins, peptides, and antibodies. It is primarily needed by professionals in biopharmaceutical R&D, diagnostic assay development, and advanced materials science for creating targeted drug conjugates, diagnostic probes, and functionalized surfaces.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via thiol-maleimide chemistry.
• Protein and Peptide Labeling: Introduction of biotin tags onto thiol-containing biomolecules for detection, purification, or immobilization on streptavidin surfaces.
• Diagnostic Assay Reagent: Fabrication of biotinylated detection probes for ELISA, lateral flow assays, and other immunoassay platforms.
• Surface Functionalization: Modification of gold nanoparticles, biosensor chips, or other materials with biotin for creating capture surfaces.
• Biomolecular Crosslinking: Covalent linkage of different biomolecules (e.g., protein-protein, protein-oligonucleotide) for structural or functional studies.
• Affinity Chromatography Ligand Synthesis: Production of biotinylated ligands for immobilization on streptavidin/avidin resins.

Basic Information

Product Name	n-Biotinyl-N'-Maleimido-Ethylenediamine
CAS No.	139554-72-6
Molecular Formula	C17H25N5O4S
Molecular Weight	395.48 g/mol
Synonyms	Biotin-PEG2-Maleimide; Biotin-PEG2-Mal; Maleimide-PEG2-Biotin; 1-Biotinamido-4-[4-(N-maleimidomethyl)cyclohexane-carboxamido]butane; N-(2-(2-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethyl)maleimide; Biotinylated Maleimide; Biotin-XX Maleimide (analogous); EZ-Link Maleimide-PEG2-Biotin (trade name type)
EINECS	Contact for details

Quality Control

Our n-Biotinyl-N'-Maleimido-Ethylenediamine is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.