Description

Ketomethylenebestatin is a potent and selective inhibitor of aminopeptidase N (APN/CD13), a zinc-dependent metalloprotease. This compound is a critical research tool for studying the role of APN in key biological processes such as angiogenesis, tumor cell invasion, and immune regulation. It is primarily utilized by researchers and developers in the pharmaceutical and biotechnology sectors for drug discovery, target validation, and biochemical pathway analysis.

Application

• Biochemical Research: A standard inhibitor for studying aminopeptidase N (APN/CD13) enzyme kinetics and function in vitro.
• Cancer Research: Investigation of APN's role in tumor angiogenesis, metastasis, and as a potential therapeutic target for anti-cancer drug development.
• Immunology Studies: Exploration of APN activity in antigen presentation, T-cell activation, and inflammatory responses.
• Neuroscience Research: Study of neuropeptide metabolism and the function of peptidases in the central nervous system.
• Pharmaceutical Screening: Used as a reference compound in high-throughput screening assays to identify novel APN inhibitors.
• Academic Research: Fundamental research in biochemistry, cell biology, and molecular pharmacology within university and institutional laboratories.

Basic Information

Product Name	Ketomethylenebestatin
CAS No.	137028-97-8
Molecular Formula	C₁₇H₂₅N₃O₄
Molecular Weight	335.40 g/mol
Synonyms	Ketomethylenebestatin; (2S)-2-[[(2S)-2-Acetamido-4-methylpentanoyl]amino]-N-[(2S)-1-hydroxy-3-methyl-1-oxobutan-2-yl]-4-methylpentanamide; APN Inhibitor; Bestatin Derivative; CD13 Inhibitor; Aminopeptidase N Inhibitor
EINECS	Contact for details

Quality Control

Our Ketomethylenebestatin is produced and tested to meet the stringent requirements of research-grade materials. Every batch undergoes comprehensive analytical characterization, including HPLC for purity, NMR and MS for structural confirmation, and specific activity testing. A detailed Certificate of Analysis (COA) is provided with each shipment, ensuring traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Biological Activity (IC₅₀)	Reported value provided

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.