Description

25 Mg Calpain Inhibitor Ii research grade is a potent, cell-permeable inhibitor of calpain proteases, specifically targeting calpain I and II. This compound is essential for researchers investigating the role of calpain in cellular processes such as apoptosis, signal transduction, and cytoskeletal remodeling. It is a critical tool for biochemical and pharmacological studies in neuroscience, cardiology, and cell biology, where precise modulation of protease activity is required.

Application

• Biochemical Research: Used as a selective inhibitor to study the enzymatic activity and physiological functions of calpain I (μ-calpain) and calpain II (m-calpain) in in vitro assays.
• Neuroscience Studies: Applied in models of neurodegenerative diseases (e.g., Alzheimer's, Parkinson's) and cerebral ischemia to investigate calpain-mediated neuronal death and axonal degeneration.
• Cardiovascular Research: Utilized to examine the role of calpain in myocardial infarction, hypertrophy, and heart failure, particularly in pathways involving protein degradation and remodeling.
• Cell Biology & Apoptosis: Employed to dissect the mechanisms of programmed cell death, where calpain activation is a key event downstream of caspase signaling.
• Muscle Biology: Used in studies of muscular dystrophy and atrophy to understand calpain's role in the degradation of cytoskeletal proteins.
• Pharmacological Screening: Serves as a reference standard or positive control in high-throughput screening campaigns for novel calpain inhibitor discovery.

Basic Information

Product Name	Calpain Inhibitor Ii (Research Grade)
CAS No.	136632-32-1
Molecular Formula	C₂₀H₃₈N₄O₄
Molecular Weight	398.54 g/mol
Synonyms	ALLN; N-Acetyl-Leu-Leu-Norleucinal; Ac-LLN-CHO; Calpain Inhibitor I; MDL 28170; Z-LLN-CHO; N-Acetyl-Leu-Leu-Nle-al; (2S)-2-Acetamido-4-methyl-N-[(2S)-1-[[(2S)-1-oxo-1-[(2S)-2-[[(2S)-2-acetamido-4-methylpentanoyl]amino]pentyl]amino]pentan-2-yl]pentanamide
EINECS	Contact for details

Quality Control

Our research-grade Calpain Inhibitor Ii is produced under stringent quality protocols. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and biological activity. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and storage recommendations is provided with every shipment to support your research reproducibility and compliance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Biological Activity (IC₅₀)	≤ 10 nM (Calpain I & II)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.