Description

2,4-Dinitrophenol-Insulin A Chain-Fluorescein Conjugate is a sophisticated, high-purity biochemical reagent designed for advanced research applications. This compound is critical for scientists requiring a highly specific and fluorescently labeled probe to study insulin-related pathways and protein interactions. It is primarily utilized by researchers and developers in the fields of pharmaceutical discovery, endocrinology, and diagnostic assay development. The conjugate offers a powerful tool for fluorescence-based detection and quantification in complex biological matrices.

Application

• Biochemical Research: Used as a fluorescent tracer in studies of insulin receptor binding, internalization, and downstream signaling pathways.
• Diagnostic Development: Serves as a key component in the development of sensitive fluorescence immunoassays (FIA) and enzyme-linked immunosorbent assays (ELISA) for insulin detection.
• Cellular Imaging: Employed in fluorescence microscopy and flow cytometry to visualize and track insulin A chain localization and dynamics within live or fixed cells.
• Drug Discovery Screening: Acts as a probe in high-throughput screening (HTS) platforms to identify novel compounds that modulate insulin-protein interactions.
• Protein-Protein Interaction Studies: Facilitates Förster resonance energy transfer (FRET) and other biophysical techniques to investigate molecular interactions involving insulin.
• Quality Control in Biopharma: Used as a reference standard in the analytical testing of insulin-based therapeutic products.

Basic Information

Product Name	2,4-Dinitrophenol-Insulin A Chain-Fluorescein Conjugate
CAS No.	134546-27-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	DNP-Insulin A Chain-FITC Conjugate; Fluorescein Isothiocyanate Labeled 2,4-Dinitrophenyl-Insulin A Chain; Insulin A Chain (DNP), FITC-labeled; FITC-Insulin A Chain-DNP; Fluorescent Insulin A Chain Derivative; 2,4-DNP-Insulin A-FITC; Insulin A Chain, Dinitrophenylated, Fluorescein Conjugate
EINECS	Contact for details

Quality Control

This high-grade biochemical is produced under strict quality control protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is characterized using advanced analytical techniques including HPLC and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, fluorescence labeling efficiency, and functional performance is provided with every shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. Allow to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, consider aliquoting to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Fluorophore-to-Protein Ratio (F/P)	1.0 - 2.0 (moles of FITC per mole of protein)
Protein Content	≥ 90% (by weight)
Solubility	Soluble in DMSO, aqueous buffers (pH >7)
Functional Activity (Binding)	Retains specific binding to anti-insulin/anti-DNP antibodies (by ELISA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.