Description

Epoxomicin is a naturally occurring, highly selective proteasome inhibitor isolated from the actinomycete *Actinoplanes utahensis*. It serves as a critical pharmacological tool for studying ubiquitin-proteasome pathway dynamics and is widely used in preclinical oncology and immunology research due to its irreversible binding mechanism and superior specificity over first-generation inhibitors like MG-132. Researchers in academic institutions, biopharmaceutical R&D labs, and contract research organizations (CROs) rely on high-purity epoxomicin for target validation, mechanism-of-action studies, and biomarker discovery. Epoxomicin CAS NO 134381-21-8 is supplied under stringent quality controls to ensure batch-to-batch reproducibility and biological activity.

Application

• Proteasome inhibition assays in human cancer cell lines (e.g., multiple myeloma, leukemia)
• Investigation of NF-κB signaling pathway regulation and apoptosis induction
• Comparative studies with bortezomib and carfilzomib to evaluate selectivity for β5 subunit
• Development of next-generation proteasome-targeted therapeutics and prodrugs
• In vivo tumor xenograft models requiring intravenous or intraperitoneal administration
• Structural biology applications including co-crystallization with 20S proteasome
• Quality control reference standard for analytical method development (HPLC, LC-MS)
• Cell-based screening platforms for identifying synergistic drug combinations

Basic Information

Product Name	Epoxomicin
CAS No.	134381-21-8
Molecular Formula	C35H55N5O9
Molecular Weight	689.85 g/mol
Synonyms	Epoxomicin; NSC 687223; UTA-1; (2S,3R,4R)-3-[(2S,3R)-3-Amino-2-hydroxy-4-methylpentanoyl]-4-[(2S,3R)-3-amino-2-hydroxy-4-methylpentanoyl]-2-[(2S,3R)-2,3-epoxybutanoyl]amino]butanoic acid; (2S,3R,4R)-3-[(2S,3R)-3-Amino-2-hydroxy-4-methylpentanoyl]-4-[(2S,3R)-3-amino-2-hydroxy-4-methylpentanoyl]-2-[(2S,3R)-2,3-epoxybutanoyl]amino]butanoic acid lactone; Epoxomicin lactone; EPOX; UT-1; Actinoplanes-derived proteasome inhibitor
EINECS	Not listed

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for identity (IR, HPLC retention time), purity (≥98.0% by HPLC), residual solvents (USP & ICH Q3C compliant), and absence of microbial contamination (bioburden testing per USP & Ph. Eur.). Testing follows validated methods aligned with ACS Reagent Grade and EP/USP general chapters for reference standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, minimize exposure to ambient humidity during handling. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.0%
Related Substances (HPLC)	Individual impurity ≤0.5%; Total impurities ≤2.0%
Residual Solvents (GC)	Meets ICH Q3C limits
Water (Karl Fischer)	≤5.0%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.