Description

Calpain Inhibitor IV is a potent, cell-permeable, and irreversible inhibitor of the calcium-dependent cysteine proteases calpain I and II. This compound is a critical research tool for studying the role of calpain activity in cellular processes such as apoptosis, signal transduction, and cytoskeletal remodeling. It is essential for researchers in pharmaceutical development, neuroscience, and cardiovascular disease research who require high-purity biochemical reagents to ensure experimental accuracy and reproducibility.

Application

• Biomedical Research: Used as a key reagent to investigate calpain-mediated pathways in models of neurodegenerative diseases (e.g., Alzheimer's, Parkinson's), muscular dystrophy, and ischemia-reperfusion injury.
• Drug Discovery & Development: Serves as a reference standard and pharmacological tool in high-throughput screening assays to identify and validate novel calpain inhibitors for therapeutic applications.
• Cell Biology Studies: Employed to inhibit calpain activity to study its role in cell migration, adhesion, proliferation, and apoptosis, providing insights into cancer metastasis and wound healing.
• Neuroscience Research: Applied in experimental models to elucidate the contribution of calpain to synaptic plasticity, long-term potentiation (LTP), and excitotoxic neuronal death.
• Biochemical Assay Development: Utilized as a control inhibitor to establish specificity and efficacy in enzymatic activity assays for calpain and related proteases.

Basic Information

Product Name	Calpain Inhibitor IV
CAS No.	133410-84-1
Molecular Formula	C₂₀H₃₈N₄O₄
Molecular Weight	398.54 g/mol
Synonyms	MDL 28170; Z-Val-Phe-CHO; Carbobenzoxy-Val-Phe-H; Calpain Inhibitor III; (S)-2-(((S)-1-((Benzyloxy)amino)-3-methyl-1-oxobutan-2-yl)carbamoyl)-N-((S)-1-oxo-3-phenylpropan-2-yl)pyrrolidine-1-carboxamide; Z-VF-CHO; N-[(Benzyloxy)carbonyl]-L-valyl-N-[(1S)-1-[[(2S,3S)-2-[[(formylamino)methyl]pentan-3-yl]amino]-3-methyl-1-oxobutan-2-yl]-L-phenylalaninamide
EINECS	Contact for details

Quality Control

Our Calpain Inhibitor IV is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the high standards required for critical research. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and reliability for your scientific work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.