Description

trans-3'-Hydroxycotinine-o-B-D-Glucuronide is a major, stable metabolite of nicotine, formed via the glucuronidation pathway. This compound is of critical importance as a high-purity reference standard and biomarker for accurate nicotine metabolism studies and toxicological analysis. It is primarily required by analytical laboratories, contract research organizations (CROs), and pharmaceutical companies engaged in pharmacokinetic research, forensic toxicology, and the development of smoking cessation therapies.

Application

• Biomarker Reference Standard for quantifying nicotine exposure and metabolism in clinical and forensic toxicology.
• Pharmacokinetic & Metabolic Research to study the disposition and clearance of nicotine and its metabolites.
• Clinical Trial Support for monitoring subject compliance and metabolic profiles in smoking cessation and nicotine-related studies.
• Method Development & Validation in analytical laboratories using LC-MS/MS, HPLC, and other chromatographic techniques.
• Forensic Analysis for definitive identification and measurement in legal and workplace testing scenarios.
• Quality Control in the manufacturing of diagnostic kits and calibrators for nicotine metabolite detection.

Basic Information

Product Name	trans-3'-Hydroxycotinine-o-B-D-Glucuronide
CAS No.	132929-88-5
Molecular Formula	C₁₆H₂₂N₂O₈
Molecular Weight	370.36 g/mol
Synonyms	trans-3'-Hydroxycotinine O-β-D-Glucuronide; (trans)-3'-Hydroxycotinine β-D-Glucuronide; 3'-Hydroxycotinine Glucuronide; Cotinine N-Oxide Glucuronide (related metabolite); Nicotine Metabolite; HOCIT-Gluc; 1-[(2S,3R,4S,5S,6R)-3,4,5-Trihydroxy-6-(hydroxymethyl)oxan-2-yl]-3-[(2R)-1-methyl-5-oxopyrrolidin-2-yl]pyridin-1-ium-2-carboxylate
EINECS	Contact for details

Quality Control

Our trans-3'-Hydroxycotinine-o-B-D-Glucuronide is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC and LC-MS/MS, to ensure high chemical purity and structural identity, meeting the stringent requirements for reference standard materials. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥95% (Area %)
Water Content (KF)	≤5.0%
Specific Optical Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.