Description

Biotin-X-D-Phe-Pro-Arg-Chloromethylketone, 2Hcl is a biotinylated, irreversible serine protease inhibitor, specifically designed for affinity purification and detection applications. This compound matters for researchers and manufacturers who require high-specificity tools for studying or targeting thrombin and related trypsin-like proteases. It is primarily needed by the pharmaceutical, biotechnology, and life science research industries for applications in proteomics, drug discovery, and diagnostic assay development.

Application

• Affinity chromatography ligand for the purification of thrombin and trypsin-like proteases.
• Biochemical research tool for studying protease inhibition kinetics and mechanisms.
• Key intermediate in the development of diagnostic kits and assays for coagulation disorders.
• Proteomics research for the identification and characterization of specific protease targets.
• Use in activity-based protein profiling (ABPP) to label active serine proteases.
• Potential component in the development of targeted therapeutic agents.

Basic Information

Product Name	Biotin-X-D-Phe-Pro-Arg-Chloromethylketone, 2Hcl
CAS No.	131104-10-4
Molecular Formula	C41H62ClN11O8S · 2HCl
Molecular Weight	~ 977.9 g/mol (free base)
Synonyms	Biotin-X-D-Phe-Pro-Arg-CH2Cl, 2HCl; Biotinylated D-Phe-Pro-Arg Chloromethyl Ketone; Biotin-D-Phe-Pro-Arg-CMK; Biotin-X-D-FPR-CMK; Thrombin Inhibitor, Biotinylated; Biotin-XX-D-Phe-Pro-Arg-CH2Cl; Biotin-XX-D-FPR-CMK; Biotin-XX-D-Phe-Pro-Arg Chloromethyl Ketone Dihydrochloride
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research and development. Each batch is characterized by HPLC, NMR, and mass spectrometry to confirm identity and purity. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.