Description

H-89 Dihydrochloride Hydrate is a potent, cell-permeable, and selective inhibitor of protein kinase A (PKA). This compound is a critical research tool for elucidating the role of PKA-mediated signaling pathways in cellular processes. It is primarily utilized by researchers and scientists in the fields of biochemistry, pharmacology, and cell biology for in vitro and in vivo studies.

Application

• Biochemical Research: A selective tool compound for studying cAMP-dependent protein kinase (PKA) activity and its downstream effects.
• Signal Transduction Studies: Used to investigate the role of PKA in various cellular signaling cascades, including those related to metabolism, gene expression, and cell proliferation.
• Pharmacological Profiling: Employed in high-throughput screening and target validation to assess the specificity of novel kinase inhibitors.
• Neuroscience Research: Applied in studies exploring PKA's function in neuronal plasticity, memory formation, and neurotransmitter release.
• Cardiovascular Research: Utilized to examine PKA's involvement in cardiac muscle contraction and vascular tone regulation.
• Cell Culture & In Vitro Assays: A standard reagent for modulating PKA activity in cultured cell lines to observe phenotypic changes.

Basic Information

Product Name	H-89 Dihydrochloride Hydrate
CAS No.	130964-39-5
Molecular Formula	C₂₀H₂₀BrN₃O₂ • 2HCl • xH₂O
Molecular Weight	Anhydrous Free Base: 422.30 g/mol
Synonyms	H-89 2HCl Hydrate; N-[2-((p-Bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide dihydrochloride hydrate; Protein Kinase A Inhibitor H-89 dihydrochloride; PKA Inhibitor H-89 2HCl; H89 2HCl Hydrate; H-89 (dihydrochloride)
EINECS	Contact for details

Quality Control

Our H-89 Dihydrochloride Hydrate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency for research applications. Each lot is accompanied by comprehensive analytical data, including HPLC purity, NMR, and mass spectrometry verification. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥98.0%
Water Content (KF)	≤5.0%
Residue on Ignition	≤0.5%
Heavy Metals	≤20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.