Description

Biotin Hpdp is a high-purity, activated biotin derivative widely used as a critical reagent in bioconjugation and labeling techniques. Its primary value lies in its specific reactivity towards sulfhydryl (-SH) groups, enabling the precise tagging of proteins, peptides, and other biomolecules for detection and purification. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable, site-specific biotinylation.

Application

• Protein & Antibody Labeling: Site-specific biotinylation of cysteine residues for use in ELISA, Western blotting, and immunoassays.
• Affinity Purification Probes: Conjugation to ligands or small molecules for pull-down assays and streptavidin/avidin-based purification systems.
• Diagnostic Kit Development: Manufacturing key components for lateral flow assays, biosensors, and other in-vitro diagnostic (IVD) devices.
• Cell Surface Tagging: Labeling of cell surface thiols for fluorescence-activated cell sorting (FACS) and microscopy studies.
• Nucleic Acid Probes: Preparation of biotinylated oligonucleotides for hybridization-based detection and genomic analysis.
• Drug Discovery & Target Identification: Creating biotinylated small molecules or peptides for target engagement and proteomics studies.

Basic Information

Product Name	Biotin Hpdp
CAS No.	129179-83-5
Molecular Formula	C25H40N6O6S2
Molecular Weight	592.75 g/mol
Synonyms	N-[6-(Biotinamido)hexyl]-3'-(2'-pyridyldithio)propionamide; Biotin-HPDP; (+)-Biotin N-hydroxysuccinimide ester; Biotinylated HPDP; 1-Biotinamido-4-[4'-(pyridyldithio)]butaneamidobutane; EZ-Link Biotin-HPDP; Sulfhydryl-reactive biotin
EINECS	Contact for details

Quality Control

Our Biotin Hpdp is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional suitability. We adhere to cGMP guidelines where applicable, and our quality commitment supports compliance in regulated pharmaceutical and diagnostic manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (Karl Fischer)	≤ 1.0%
Functional Activity	Conforms (by sulfhydryl coupling assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.