Description

1,25-Dihydroxyvitamin D3-[D3] (Certimass Solution) is a high-purity, isotopically labeled internal standard solution, specifically designed for precise quantitative analysis in mass spectrometry. This compound is critical for ensuring accuracy and reliability in bioanalytical research and pharmaceutical quality control by correcting for instrument variability and matrix effects. It is an essential tool for researchers and quality assurance professionals in pharmaceutical development, clinical diagnostics, and nutritional science who require definitive measurement of vitamin D metabolites.

Application

• Internal Standard for LC-MS/MS: Primary use as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for the analysis of 1,25-dihydroxyvitamin D3 and related metabolites in biological matrices.
• Clinical Research & Diagnostics: Enables accurate measurement of active vitamin D levels in serum and plasma for studies on bone metabolism, renal function, and endocrine disorders.
• Pharmaceutical Bioanalysis: Supports pharmacokinetic and pharmacodynamic studies for drug development targeting vitamin D pathways.
• Method Development & Validation: Serves as a critical component in developing, optimizing, and validating robust analytical methods in compliance with regulatory guidelines (e.g., FDA, EMA).
• Nutritional Science Studies: Used in research to precisely quantify vitamin D status and metabolism in nutritional interventions.
• Quality Control Laboratories: Ensures the accuracy and precision of in-house testing methods within pharmaceutical and contract research organizations.

Basic Information

Product Name	1,25-Dihydroxyvitamin D3-[D3] (Certimass Solution)
CAS No.	128723-16-0
Molecular Formula	C27H40D3O3
Molecular Weight	Approx. 419.65 g/mol
Synonyms	Calcitriol-d3; 1,25-Dihydroxycholecalciferol-d3; 1α,25-Dihydroxyvitamin D3-d3; (5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-1α,3β,25-triol-d3; 1,25(OH)2D3-d3; 1,25-Dihydroxyvitamin D3 Internal Standard; Rocaltrol-d3 (labeled analog)
EINECS	Contact for details

Quality Control

Our 1,25-Dihydroxyvitamin D3-[D3] (Certimass Solution) is manufactured under strict quality systems to ensure the highest standards of purity and isotopic enrichment required for analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as chemical and isotopic purity, concentration, and solvent verification. We adhere to industry best practices suitable for research and development use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated). Due to its highly volatile nature, ensure containers are securely sealed to prevent solvent evaporation and concentration changes. For long-term stability, consider storage at -20°C or below.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (HPLC/MS)	Conforms to reference standard
Assay (Concentration)	Typically 1 µg/mL (or as specified)
Chemical Purity (HPLC)	≥ 98%
Isotopic Purity (D3)	≥ 98 atom % D
Solvent	Methanol, Ethanol, or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.