Description

Hirudin (54-64), N(Alpha)-Dinitrofluorobenzyl- is a chemically modified peptide derivative of the potent natural thrombin inhibitor, hirudin. This specific derivative is a critical research-grade biochemical tool used for studying serine protease inhibition mechanisms and developing novel anticoagulant therapies. It is primarily utilized by pharmaceutical R&D departments, academic research institutions, and biotechnology companies focused on cardiovascular disease and hemostasis. The compound offers high specificity for thrombin, making it invaluable for structure-activity relationship (SAR) studies and biochemical assay development.

Application

• Biochemical Research: As a high-affinity, site-directed inhibitor for mechanistic studies of thrombin and related serine proteases.
• Pharmaceutical Development: Serving as a reference standard or lead compound in the discovery and development of new anticoagulant and antithrombotic drugs.
• Diagnostic Reagent Formulation: Used in the development and calibration of in-vitro diagnostic kits for measuring thrombin activity or clotting parameters.
• Proteomics & Enzyme Kinetics: Employed in detailed kinetic analyses to determine inhibition constants (Ki) and understand enzyme-inhibitor interactions.
• Academic Studies: A key reagent in university laboratories for teaching and advanced research in biochemistry, hematology, and pharmacology.

Basic Information

Item	Details
Product Name	Hirudin (54-64), N(Alpha)-Dinitrofluorobenzyl-
CAS No.	127761-72-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	N(Alpha)-Dinitrofluorobenzyl-Hirudin (54-64); Dinitrofluorobenzyl-Hirudin Fragment 54-64; Hirudin Peptide (54-64) DNFB Derivative; [DNFB]Hirudin54-64; Thrombin Inhibitor Peptide Derivative; Fmoc-chemistry derived hirudin analog; N-α-(2,4-Dinitro-5-fluorophenyl)-hirudin (54-64)
EINECS	Contact for details

Quality Control

Our Hirudin (54-64), N(Alpha)-Dinitrofluorobenzyl- is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and reliability for critical research applications. Each lot is characterized using advanced analytical techniques, including HPLC for purity assessment and mass spectrometry for identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and other relevant parameters is provided with every shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated freezer. The product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (Peptide Content)	≥ 80%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.