Description

7-Ketocholesterol-25,26,26,26,27,27,27-D7 is a high-purity, deuterium-labeled analog of the oxysterol 7-ketocholesterol, a key biomarker of oxidative stress and cholesterol autoxidation. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring analytical accuracy and reproducibility. It is primarily utilized by research institutions, pharmaceutical companies, and clinical diagnostic laboratories for lipidomics research, metabolic disorder studies, and the development of advanced analytical methods.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a critical internal standard for the precise quantification of endogenous 7-ketocholesterol in biological matrices such as plasma, serum, and tissues.
• Biomarker Research: Enables accurate measurement of 7-ketocholesterol levels in studies of atherosclerosis, neurodegenerative diseases (e.g., Alzheimer's), diabetes, and other conditions linked to oxidative stress.
• Lipidomics and Metabolomics: A vital tool in high-throughput profiling of oxysterols and lipid oxidation products to understand metabolic pathways and disease mechanisms.
• Drug Development & Pharmacology: Used in pharmacokinetic and pharmacodynamic studies to monitor drug effects on cholesterol metabolism and oxidative stress pathways.
• Clinical Diagnostics: Supports the development and validation of diagnostic assays for oxidative stress markers.
• Food & Nutritional Science: Applied in research analyzing cholesterol oxidation products (COPs) in foodstuffs to assess nutritional quality and safety.

Basic Information

Product Name	7-Ketocholesterol-25,26,26,26,27,27,27-D7
CAS No.	127684-08-6
Molecular Formula	C₂₇H₃₉D₇O₃
Molecular Weight	413.70 g/mol
Synonyms	7-Ketocholesterol-d7; (3β)-Cholest-5-en-3-ol-7-one-25,26,26,26,27,27,27-d7; 7-Oxocholesterol-d7; 7-KC-d7; 5-Cholesten-3β-ol-7-one-d7; 7-Dehydrocholesterol-7-one-d7; 3β-Hydroxy-5-cholesten-7-one-d7
EINECS	Contact for details

Quality Control

Our 7-Ketocholesterol-25,26,26,26,27,27,27-D7 is manufactured under strict quality protocols to ensure the high chemical and isotopic purity required for analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and mass spectrometry, to confirm identity, purity, and deuterium enrichment. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. For long-term storage, desiccate to protect against moisture. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Solubility	Soluble in chloroform, DMSO, ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.