Description

Biotin-Phenylalanyl-Alanine Diazomethyl Ketone is a specialized biotinylated peptide derivative designed for targeted protease inhibition research. This compound is a crucial tool for studying enzyme kinetics and developing selective inhibitors, particularly in the field of proteomics and drug discovery. It is primarily utilized by pharmaceutical R&D teams, academic biochemistry laboratories, and biotechnology companies focused on enzyme mechanism elucidation and therapeutic target validation.

Application

• Protease Inhibition Studies: Acts as an active site-directed, irreversible inhibitor for mechanistic studies of serine and cysteine proteases.
• Affinity Labeling & Probe Development: The biotin tag enables efficient isolation, detection, and purification of target enzymes or enzyme-inhibitor complexes using streptavidin-based systems.
• Biochemical Assay Development: Used in high-throughput screening (HTS) assays to identify and characterize novel protease inhibitors.
• Proteomics Research: Employed for the specific labeling and identification of protease activity in complex biological samples.
• Drug Discovery & Target Validation: Serves as a key intermediate or pharmacological tool in the development pathway for therapeutic protease inhibitors.
• Enzyme Structure-Function Analysis: Aids in mapping active sites and understanding substrate specificity through covalent modification.

Basic Information

Product Name	Biotin-Phenylalanyl-Alanine Diazomethyl Ketone
CAS No.	127678-91-5
Molecular Formula	C26H36N6O5S
Molecular Weight	544.67 g/mol
Synonyms	Biotin-Phe-Ala-CHN2; Biotinyl-Phenylalanyl-Alanyl Diazomethane; Biotin-Phe-Ala Diazomethyl Ketone; Bz-Phe-Ala-CHN2 (Biotin conjugate); (S)-5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(1-((S)-1-((diazomethyl)amino)-3-phenyl-1-oxopropan-2-yl)amino)-1-oxo-3-phenylpropan-2-yl)pentanamide; Biotinylated Peptide Diazomethyl Ketone; Protease Inhibitor, Biotinylated.
EINECS	Contact for details

Quality Control

Our Biotin-Phenylalanyl-Alanine Diazomethyl Ketone is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and MS and NMR for structural confirmation, to ensure consistency and reliability for critical research applications. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.