Description

Isoleucyl-Phenylalanyl-Lysine Chloromethyl Ketone is a synthetic tripeptide chloromethyl ketone derivative, a potent and irreversible inhibitor of serine proteases. This compound is critical for biochemical research and pharmaceutical development, enabling precise study and modulation of enzymatic activity. It is primarily utilized by researchers in proteomics, enzymology, and medicinal chemistry, as well as by manufacturers developing therapeutic agents targeting specific proteolytic pathways.

Application

• Biochemical Research: As a specific, irreversible inhibitor for studying the mechanism and kinetics of serine proteases like trypsin, plasmin, and related enzymes.
• Proteomics & Target Validation: Used to selectively inhibit target proteases in complex biological samples to elucidate their functional roles in cellular pathways.
• Pharmaceutical Development: Serves as a key pharmacophore or lead compound in the design of novel therapeutic agents for conditions involving dysregulated proteolysis (e.g., thrombosis, inflammation, cancer).
• Enzyme Characterization: Employed to determine active site specificity and to affinity-label serine proteases for structural analysis.
• Diagnostic Reagent Formulation: Potential use in the development of diagnostic kits and assays that require controlled inhibition of specific proteolytic enzymes.

Basic Information

Product Name	Isoleucyl-Phenylalanyl-Lysine Chloromethyl Ketone
CAS No.	126642-86-2
Molecular Formula	C26H41ClN4O5
Molecular Weight	525.08 g/mol
Synonyms	Ile-Phe-Lys-CH2Cl; IFK-CMK; Isoleucine-Phenylalanine-Lysine Chloromethyl Ketone; Ac-Ile-Phe-Lys-CH2Cl; N-Acetyl-Isoleucyl-Phenylalanyl-Lysyl Chloromethyl Ketone; Protease Inhibitor IFK-CMK; TPCK-related Tripeptide Inhibitor; Serine Protease Inhibitor (Ile-Phe-Lys)
EINECS	Contact for details

Quality Control

Our Isoleucyl-Phenylalanyl-Lysine Chloromethyl Ketone is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via mass spectrometry and NMR, to ensure it meets the high standards required for research and development. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to reach room temperature before opening to prevent condensation and moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (Titration)	≥ 98%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.