Description

Cholesteryl Oleate, [Oleate-9,10-3H] is a high-specific-activity, tritium-labeled derivative of cholesteryl oleate, a key cholesteryl ester. This radiolabeled compound is an essential tool for tracing and quantifying metabolic pathways involving cholesterol esterification, transport, and storage in biological systems. It is primarily used by research scientists and pharmaceutical developers in lipid metabolism studies, atherosclerosis research, and drug discovery programs targeting lipid disorders.

Application

• Lipid Metabolism & Atherosclerosis Research: As a tracer to study the uptake, esterification, and efflux of cholesterol in macrophages, hepatocytes, and other cell types relevant to foam cell formation.
• Drug Discovery & Screening: Used in high-throughput assays to evaluate the efficacy of novel therapeutic compounds targeting enzymes like acyl-CoA:cholesterol acyltransferase (ACAT) or lecithin–cholesterol acyltransferase (LCAT).
• Lipoprotein Metabolism Studies: Tracing the incorporation and turnover of cholesteryl esters within lipoproteins (e.g., LDL, HDL) to understand reverse cholesterol transport and other pathways.
• Biochemical & Enzymatic Assays: Serving as a specific substrate for in vitro assays to measure the activity of cholesterol-esterifying or hydrolyzing enzymes.
• Autoradiography & Imaging: Enabling the visualization of cholesteryl ester localization and accumulation in tissue sections or cell cultures.
• Absorption & Biodistribution Studies: Investigating the intestinal absorption, tissue distribution, and pharmacokinetics of dietary or endogenous cholesteryl esters in animal models.

Basic Information

Product Name	Cholesteryl Oleate, [Oleate-9,10-3H]
CAS No.	125372-16-9
Molecular Formula	C45H78O2 (unlabeled parent compound)
Molecular Weight	651.11 g/mol (unlabeled parent compound)
Synonyms	Cholesteryl (9,10-3H)oleate; (9,10-3H)Cholesteryl Oleate; 3β-Cholest-5-en-3-yl (9Z)-9-octadecenoate-9,10-3H; Tritiated Cholesteryl Oleate; [9,10-3H]-Cholesteryl Oleate; Cholesterol Oleate-9,10-3H; Radiolabeled Cholesteryl Oleate
EINECS	Contact for details

Quality Control

Every batch of our radiolabeled compounds is produced and tested to meet the exacting standards required for life science research. Rigorous analytical controls, including HPLC and radio-HPLC, are employed to ensure high chemical and radiochemical purity, as well as specific activity. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity (Ci/mmol or GBq/mmol), solvent, and analytical methods is provided with each shipment to ensure traceability and reproducibility in your experiments.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store the solution at or below -20°C. Store in accordance with all local, national, and international regulations governing the handling and disposal of radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC)	Conforms to reference standard
Chemical Purity (HPLC)	≥ 98%
Radiochemical Purity (Radio-HPLC)	≥ 97%
Specific Activity	30-60 Ci/mmol (1.11-2.22 TBq/mmol) [Custom activities available]
Concentration	1.0 mCi/mL (37 MBq/mL) in ethanol or toluene [Custom solvents/concentrations available]
Solvent	As specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.