Description

Timp-2, Human is a highly purified, recombinant protein belonging to the tissue inhibitor of metalloproteinases (TIMP) family, specifically inhibiting matrix metalloproteinase-2 (MMP-2). This compound is essential for researchers studying extracellular matrix remodeling, tissue repair, and the pathophysiology of diseases such as cancer, fibrosis, and cardiovascular disorders. It is a critical reagent for biochemical assays, cell culture studies, and the development of therapeutic strategies targeting MMP activity in academic, pharmaceutical, and biotechnology sectors globally.

Application

• Biochemical Research: Used as a specific inhibitor in enzymatic assays to study MMP-2 kinetics, substrate specificity, and inhibition mechanisms.
• Cell Culture & In Vitro Studies: Applied to modulate extracellular matrix degradation in cell-based models of invasion, migration, and angiogenesis.
• Drug Discovery & Development: Serves as a reference standard and tool compound for screening and validating novel MMP-2 inhibitors.
• Disease Mechanism Investigation: Utilized in research models to elucidate the role of TIMP-2/MMP-2 balance in cancer progression, tissue fibrosis, and inflammatory diseases.
• Diagnostic Development: May be employed as a calibrator or component in immunoassays designed to measure TIMP-2 levels in clinical samples.
• Protein-Protein Interaction Studies: Used to investigate the binding interactions between TIMP-2 and various MMPs or cell surface receptors.

Basic Information

Product Name	Timp-2, Human
CAS No.	124861-55-8
Molecular Formula	C~220H~340N~60O~70S~10 (Approx., recombinant protein)
Molecular Weight	~21.4 kDa (non-glycosylated form)
Synonyms	TIMP-2; TIMP2; Tissue Inhibitor of Metalloproteinases 2, Human Recombinant; CSC-21K; DDC8; TIMP Metallopeptidase Inhibitor 2; TIMP-2 Human; Recombinant Human TIMP-2; TIMP2 Protein, Human
EINECS	Contact for details

Quality Control

Our recombinant Timp-2, Human is produced under stringent conditions and undergoes comprehensive analytical testing to ensure high purity, identity, and biological activity. Each batch is tested for endotoxin levels to ensure suitability for cell-based applications. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data on purity (by SDS-PAGE and HPLC), protein concentration, biological activity (MMP-2 inhibition assay), and sterility where applicable.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, it is recommended to store lyophilized protein at -20°C or below. Reconstituted solutions should be aliquoted and stored at -80°C to avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band at ~21 kDa under reducing conditions
Purity (HPLC)	≥ 95%
Protein Content	≥ 90% (by UV absorbance)
Biological Activity	≥ 1 x 104 units/mg (MMP-2 inhibition assay)
Endotoxin Level	< 1.0 EU/µg (LAL assay)
Solubility	Soluble in water or PBS, pH 7.2-7.4

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.