Description

8-((4-Bromo-2,3-Dioxobutyl)Thio)-Adenosine 3',5'-Cyclic Monophosphate is a chemically modified derivative of cyclic adenosine monophosphate (cAMP), a fundamental secondary messenger in cellular signaling. This compound is specifically engineered as a potent and cell-permeable activator of protein kinase A (PKA), making it a critical tool for probing intracellular signal transduction pathways. It is primarily utilized by researchers in biochemistry, molecular biology, and pharmacology for studying cellular responses, enzyme regulation, and drug discovery mechanisms.

Application

• Biochemical Research: As a high-affinity, membrane-permeable activator of Protein Kinase A (PKA) for studying cAMP-dependent signaling cascades in vitro and in cell-based assays.
• Pharmaceutical Development: Used as a reference standard and pharmacological tool compound in drug discovery programs targeting PKA-related disorders, such as certain cancers and metabolic diseases.
• Cell Signaling Studies: Investigation of the role of cAMP and PKA in processes like gene expression, metabolism, ion channel function, and cell proliferation/differentiation.
• Enzyme Kinetics: Employed in enzymatic assays to characterize PKA activity, inhibition, and substrate specificity under controlled experimental conditions.
• Neuroscience Research: Exploration of synaptic plasticity, neurotransmitter release, and long-term potentiation (LTP) where PKA signaling plays a crucial role.
• Diagnostic Reagent Manufacturing: Potential use as a key component in the development of diagnostic kits and assays for measuring PKA activity in clinical samples.

Basic Information

Product Name	8-((4-Bromo-2,3-Dioxobutyl)Thio)-Adenosine 3',5'-Cyclic Monophosphate
CAS No.	124357-34-2
Molecular Formula	C₁₄H₁₅BrN₅O₈PS
Molecular Weight	548.24 g/mol
Synonyms	8-Bromo-cAMP; 8-Br-cAMP; 8-Bromoadenosine 3',5'-cyclic monophosphate; 8-Bromo-cyclic AMP; 8-Br-cyclic adenosine monophosphate; 8-Bromoadenosine cyclic 3',5'-(hydrogen phosphate); 8-(4-Bromo-2,3-dioxobutylthio)adenosine 3',5'-cyclic monophosphate
EINECS	Contact for details

Quality Control

Our 8-((4-Bromo-2,3-Dioxobutyl)Thio)-Adenosine 3',5'-Cyclic Monophosphate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and verification of biological activity. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Our quality commitment supports reproducible and reliable results for your critical experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, keep desiccated. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.