Description

L-γ-Glutamyl-p-Nitroanilide Monohydrate is a high-purity chromogenic substrate specifically designed for enzymatic assays. This compound is critical for the accurate and sensitive determination of γ-glutamyltransferase (GGT) activity, a key diagnostic enzyme. It is an essential reagent for clinical diagnostics, pharmaceutical research, and biochemical studies where precise enzyme kinetics are measured.

Application

• Clinical Diagnostic Reagent: Primary substrate in in vitro diagnostic (IVD) kits for the quantitative colorimetric assay of serum γ-Glutamyl Transferase (GGT), a vital liver and biliary tract function marker.
• Biochemical Research: Used in enzyme kinetics studies, inhibition assays, and purification processes to characterize GGT activity from various biological sources.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and evaluate potential therapeutic agents that modulate GGT activity.
• Quality Control in Manufacturing: Serves as a reference standard for calibrating and validating diagnostic equipment and reagent lots in medical device and IVD kit production.
• Food & Beverage Analysis: Applied in specialized testing protocols to monitor microbial activity or specific enzymatic processes in industrial fermentation.

Basic Information

Product Name	L-γ-Glutamyl-p-Nitroanilide Monohydrate
CAS No.	122864-94-2
Molecular Formula	C11H14N4O6 • H2O
Molecular Weight	304.26 g/mol (Monohydrate)
Synonyms	L-γ-Glutamyl-p-nitroanilide monohydrate; γ-Glutamyl-p-Nitroanilide; GGPNA; GGPN; L-Glutamic acid γ-(p-nitroanilide) monohydrate; 5-L-Glutamyl-4-nitroanilide monohydrate; N-(4-Nitrophenyl)-L-glutamine γ-p-nitroanilide monohydrate; GGT Substrate
EINECS	Contact for details

Quality Control

Every batch of L-γ-Glutamyl-p-Nitroanilide Monohydrate is manufactured and tested under a strict quality management system. We ensure compliance with critical parameters for diagnostic and research applications, including purity, absorbance characteristics, and enzymatic suitability. A comprehensive Certificate of Analysis (COA) detailing identity, assay, impurity profile, and performance data is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture absorption, which can affect stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange-yellow crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	5.0% - 7.0% (Theoretical for monohydrate: ~5.9%)
Absorbance (Lambda max)	310 - 322 nm (in suitable solvent)
Enzymatic Suitability	Suitable for GGT assay
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.