Description

Dolastatin 13 is a naturally derived cytotoxic pentapeptide originally isolated from the sea hare *Dolabella auricularia*, recognized for its potent microtubule-depolymerizing activity and role as a key structural template in antibody–drug conjugate (ADC) development. It serves as a critical reference standard and synthetic intermediate for pharmaceutical R&D, particularly in oncology-focused biotech and contract development and manufacturing organizations (CDMOs). Researchers and process chemists requiring high-integrity biochemical tools for preclinical target validation, assay calibration, and structure–activity relationship (SAR) studies rely on rigorously characterized Dolastatin 13 CAS NO 120231-23-4 to ensure reproducibility and regulatory traceability.

Application

• Lead compound and structural scaffold for next-generation antimitotic ADC payloads (e.g., analogues of monomethyl auristatin E/F)
• Reference standard in HPLC and LC-MS/MS analytical method development and validation for cytotoxic peptide quantification
• Tool compound in mechanistic cell biology studies investigating tubulin dynamics, mitotic arrest, and apoptosis induction
• Intermediate in the multi-step synthesis of clinical-stage auristatin derivatives used in FDA-approved and investigational ADCs
• Calibration material for potency assays in GMP-compliant quality control laboratories supporting biologics manufacturing
• Research-grade reagent for high-throughput screening of tubulin-binding agents in academic and industrial discovery programs

Basic Information

Product Name	Dolastatin 13
CAS No.	120231-23-4
Molecular Formula	C57H84N12O15S
Molecular Weight	1217.43 g/mol
Synonyms	Dolastatin-13; Dolastatin 13; NSC 376128; (3S,6S,9S,12S,15S,18S,21S,24S,27S,30S,33S,36S)-3,6,9,12,15,18,21,24,27,30,33,36-Dodecamethyl-1,4,7,10,13,16,19,22,25,28,31,34-dodecaoxo-2,5,8,11,14,17,20,23,26,29,32,35-dodecaaza-cyclohexatriaconta-1(36),2(35),4(34),5(33),7(32),8(31),10(30),11(29),13(28),14(27),16(26),17(25),19(24),20(23),22(21),23(20),25(19),26(18),28(17),29(16),31(15),32(14),34(13),35(12)-tetracontatetraene; Dolastatin XIII; Dolastatin-13 (natural)
EINECS	Not listed

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for identity (HPLC retention time match, IR spectral overlay), purity (≥98.5% by HPLC-UV), residual solvents (ICH Q3C compliant), heavy metals (<10 ppm), and microbiological limits (where applicable). Testing adheres to ICH Q5, Q6B, and USP <1058> guidelines for analytical instrument qualification.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, maintain in a low-humidity environment (<30% RH) and minimize exposure to ambient air during handling.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time matches reference standard (±0.2 min)
Identification (IR)	FT-IR spectrum conforms to reference
Assay (HPLC-UV)	≥98.5%
Related Substances (HPLC)	Single impurity ≤0.5%; Total impurities ≤1.5%
Residual Solvents (GC)	Meets ICH Q3C Class 3 limits
Water Content (KF)	≤5.0%
Heavy Metals	<10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.