Description

Placental Ribonuclease Inhibitor is a highly purified, potent protein that specifically inhibits the activity of ribonucleases (RNases). This product is critical for protecting the integrity of RNA in sensitive molecular biology applications, preventing unwanted degradation that can compromise experimental results. It is an essential reagent for researchers and manufacturers in molecular biology, diagnostics, and biopharmaceutical development, where high-quality RNA is paramount.

Application

• RNA Isolation and Purification: Added to lysis buffers to protect RNA from endogenous RNases during extraction from tissues, cells, or biological fluids.
• cDNA Synthesis (Reverse Transcription): Essential for first-strand cDNA synthesis reactions to prevent RNase H and other RNases from degrading the RNA template.
• In Vitro Transcription/Translation: Used in cell-free protein expression systems to safeguard mRNA templates from degradation.
• Long-term RNA Storage: Incorporated into RNA storage solutions to maintain sample integrity over extended periods at -20°C or -80°C.
• Diagnostic Assay Development: Employed in the formulation of master mixes for RT-PCR and other nucleic acid amplification tests (NAATs) to ensure assay reliability and sensitivity.
• Biopharmaceutical Manufacturing: Used in processes involving mRNA-based therapeutics and vaccines to control RNase activity during production and purification.

Basic Information

Item	Details
Product Name	Placental Ribonuclease Inhibitor
CAS No.	120178-77-0
Molecular Formula	Contact for details
Molecular Weight	~50 kDa (Monomer)
Synonyms	RNase Inhibitor; Ribonuclease Inhibitor; Human Placental Ribonuclease Inhibitor; RNasin® (Trade Name); Rnh1; RNI; Ribonuclease Inactivator; Angiogenin Inhibitor
EINECS	Contact for details

Quality Control

Our Placental Ribonuclease Inhibitor is produced under stringent conditions to ensure the highest level of activity and purity. Each batch undergoes rigorous quality testing, including functional activity assays and purity analysis, to guarantee performance in critical applications. Certificates of Analysis (COA) detailing purity, specific activity, and absence of contaminating nucleases are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (SDS-PAGE)	Single band corresponding to ~50 kDa
Purity (HPLC)	≥ 95%
Specific Activity	≥ 5,000 units/µL
Protein Concentration	Provided on COA
DNase Activity	Not detectable
Protease Activity	Not detectable
pH	7.0 - 8.0
Buffer	50 mM Tris-HCl, 50% Glycerol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.