Description

5-Acetamido-9-(3-Fluoresceinylthioureido)-3,5,9-Trideoxy-2-Nonulosonsonic Acid is a specialized, high-purity biochemical reagent designed for advanced research and diagnostic applications. This compound is critical for scientists and manufacturers requiring precise, fluorescently-labeled sialic acid derivatives for sensitive detection and analysis. It is primarily utilized by professionals in the fields of glycobiology research, diagnostic assay development, and pharmaceutical discovery, where its unique structure enables the study of carbohydrate-protein interactions and cellular processes.

Application

• Glycobiology Research: Used as a key substrate or probe for studying sialic acid metabolism, enzyme kinetics (e.g., sialidases, sialyltransferases), and glycan biosynthesis pathways.
• Diagnostic Assay Development: Serves as a critical component in the development of high-sensitivity fluorescent immunoassays (FIAs) and enzyme-linked immunosorbent assays (ELISAs) for detecting specific antibodies or antigens.
• Pharmaceutical Screening: Employed in drug discovery platforms to identify and characterize inhibitors or modulators of sialic acid-related enzymes and receptors.
• Cell Biology and Imaging: Functions as a fluorescent tracer for labeling cell surface glycoconjugates, enabling visualization and analysis of sialic acid expression and localization via fluorescence microscopy or flow cytometry.
• Bioconjugation and Probe Synthesis: Acts as a versatile intermediate for synthesizing more complex fluorescent probes, affinity labels, or bioconjugates targeting specific biological structures.
• Quality Control Reference Standard: Provides a certified reference material for the quality control and validation of diagnostic kits and research reagents in regulated environments.

Basic Information

Product Name	5-Acetamido-9-(3-Fluoresceinylthioureido)-3,5,9-Trideoxy-2-Nonulosonsonic Acid
CAS No.	118694-47-6
Molecular Formula	C32H33N3O12S
Molecular Weight	683.68 g/mol
Synonyms	Fluoresceinylthioureido-Sialic Acid; 5-Acetamido-9-[(3-fluoresceinyl)thioureido]-3,5,9-trideoxy-D-glycero-D-galacto-non-2-ulopyranosonic Acid; Fluorescein-labeled Sialic Acid; Sialic Acid-Fluorescein Conjugate; 9-(3-Fluoresceinylthioureido)-5-acetamido-3,5,9-trideoxy-D-glycero-D-galacto-non-2-ulopyranosonic Acid; F-SA; Contact for additional aliases.
EINECS	Contact for details

Quality Control

Our production of 5-Acetamido-9-(3-Fluoresceinylthioureido)-3,5,9-Trideoxy-2-Nonulosonsonic Acid adheres to stringent quality management protocols to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and spectrophotometry for fluorescence characteristics. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications. We support compliance with GMP and ISO 9001 standards for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive); ensure the container is sealed under an inert atmosphere or with desiccant to maintain stability. Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 3.0%
Fluorescence Properties	Ex/Em: ~494 nm / ~521 nm (verify in buffer)
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.