Description

Bafilomycin A CAS NO 116764-51-3 is a potent and highly specific macrolide antibiotic that functions as a vacuolar-type H+-ATPase (V-ATPase) inhibitor. This compound is a critical research tool for studying intracellular vesicle trafficking, autophagy, and lysosomal function. It is essential for researchers and developers in the fields of cell biology, pharmacology, and oncology, providing a reliable mechanism to probe cellular processes and develop novel therapeutic strategies.

Application

• Biomedical Research: A fundamental tool for investigating autophagy, lysosomal acidification, and endocytic pathways in cell culture models.
• Pharmacology & Drug Discovery: Used as a reference standard and positive control in screening assays for novel V-ATPase inhibitors and autophagy modulators.
• Cancer Research: Employed to study tumor cell metabolism, drug resistance mechanisms, and to induce apoptosis in various cancer cell lines.
• Microbiology: Used in studies of bacterial toxin entry and intracellular pathogen survival mechanisms that rely on host cell vesicular transport.
• Neuroscience: Applied in research on neurotransmitter release and synaptic vesicle recycling processes.
• Biochemical Assay Development: Serves as a key component in establishing and validating in vitro enzyme activity assays for V-ATPase.

Basic Information

Product Name	Bafilomycin A
CAS No.	116764-51-3
Molecular Formula	C35H58O9
Molecular Weight	622.83 g/mol
Synonyms	Bafilomycin A1; BafA1; Bafilomycin A from *Streptomyces griseus*; V-ATPase Inhibitor; NSC 381509; Antibiotic X 14885A; Antibiotic X-14885A; 3,9-Dihydroxy-14-[(1R,2S,3S,4S)-1,2,3,4,5-pentahydroxy-6-methylheptyl]-4,10-dimethoxy-8-methyl-1,6-dioxacyclotetradecane-2,7,12-trione
EINECS	Contact for details

Quality Control

Our Bafilomycin A is produced under strict quality management protocols to ensure batch-to-batch consistency and high biological activity. Each lot is characterized using advanced analytical techniques, including HPLC, NMR, and MS, to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, potency, and analytical results is provided with every shipment to support your research integrity and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Biological Activity (IC50)	≤ 10 nM (V-ATPase inhibition)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.