Description

N(Alpha)-((Acetylthio)Acetyl)-Phenylalanyl-Prolyl-Arginine Chloromethyl Ketone is a potent and selective synthetic peptide-based inhibitor designed to target specific serine proteases. This compound matters for researchers and developers requiring high-purity, well-characterized biochemical tools for precise enzymatic studies and inhibitor design. It is primarily needed by pharmaceutical R&D teams, academic biochemistry laboratories, and diagnostic kit manufacturers focused on protease-related pathways, coagulation cascades, and inflammatory processes.

Application

• Biochemical Research: Used as a selective active-site titrant and irreversible inhibitor in mechanistic studies of serine proteases like thrombin, trypsin, and related enzymes.
• Pharmaceutical Development: Serves as a key reference standard and pharmacophore model in the discovery and optimization of novel anticoagulant and anti-inflammatory drug candidates.
• Diagnostic Reagent Manufacturing: Employed in the calibration and quality control of diagnostic assays that measure protease activity, particularly in hemostasis and thrombosis testing panels.
• Enzymology Studies: Facilitates the determination of enzyme kinetics, inhibition constants (Ki), and the study of enzyme-inhibitor complex formation under controlled conditions.
• Cell Biology & Proteomics: Applied in cellular models to selectively inhibit specific protease activities, helping to elucidate their functional roles in signaling pathways and disease states.

Basic Information

Product Name	N(Alpha)-((Acetylthio)Acetyl)-Phenylalanyl-Prolyl-Arginine Chloromethyl Ketone
CAS No.	115290-74-9
Molecular Formula	C28H41N7O6S
Molecular Weight	603.73 g/mol
Synonyms	Ac-(S-CH2CO)-Phe-Pro-Arg-CH2Cl; Acetylthioacetyl-Phe-Pro-Arg-chloromethyl ketone; ATAP-PRACK; Thrombin Inhibitor II, TAPCK analog; Nα-[(Acetylthio)acetyl]-L-phenylalanyl-N-[(3S)-1-(N2-{N-[(dichloromethylene)(imino)methyl]-L-ornithyl}-L-arginyl)-2-oxopyrrolidin-3-yl]carboxamide; (Acetylthio)acetyl-Phe-Pro-Arg-CH2Cl; APC-366; Serine Protease Inhibitor (Arg derivative)
EINECS	Contact for details

Quality Control

Our N(Alpha)-((Acetylthio)Acetyl)-Phenylalanyl-Prolyl-Arginine Chloromethyl Ketone is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and potency. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing specifications such as HPLC purity, chiral purity, water content, and residual solvents. We adhere to high-purity peptide standards suitable for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Potency (Enzymatic Assay)	≥ 90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.