Description

n-Acetyl-Leucyl-Phenylalanyl Trifluoromethyl Ketone is a specialized synthetic peptide derivative designed as a potent and selective inhibitor of proteolytic enzymes, particularly those in the serine protease family. This compound is a critical research tool for studying enzyme kinetics, substrate specificity, and developing therapeutic strategies for conditions involving dysregulated proteolysis. It is primarily utilized by pharmaceutical R&D teams, academic biochemists, and contract research organizations focused on enzymology and drug discovery.

Application

• Enzyme Inhibition Studies: Serves as a key biochemical tool for investigating the mechanism and kinetics of serine proteases like chymotrypsin, elastase, and related enzymes.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) assays and as a lead compound or reference standard in the development of novel protease-targeted therapeutics.
• Biochemical Pathway Analysis: Employed to selectively inhibit specific proteases to elucidate their role in complex biological pathways, including inflammation, blood coagulation, and apoptosis.
• Proteomics Research: Acts as an affinity ligand or activity-based probe for the identification, purification, and characterization of protease activity in complex biological samples.
• Academic & Contract Research: A fundamental reagent for universities and CROs conducting basic research in biochemistry, molecular biology, and medicinal chemistry.

Basic Information

Product Name	n-Acetyl-Leucyl-Phenylalanyl Trifluoromethyl Ketone
CAS No.	113215-69-3
Molecular Formula	C₂₂H₃₀F₃N₃O₅
Molecular Weight	473.49 g/mol
Synonyms	Ac-Leu-Phe-CF₃; N-Acetyl-L-leucyl-L-phenylalanyl trifluoromethyl ketone; Acetylleucylphenylalanyl trifluoromethyl ketone; Protease Inhibitor Ac-Leu-Phe-CF₃; Chymotrypsin Inhibitor Ac-Leu-Phe-CF₃; ALPF-CF₃; (2S)-2-Acetamido-N-[(2S)-1-oxo-3-phenyl-1-[(2,2,2-trifluoroacetyl)amino]propan-2-yl]-4-methylpentanamide
EINECS	Contact for details

Quality Control

Every batch of n-Acetyl-Leucyl-Phenylalanyl Trifluoromethyl Ketone is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure identity, purity, and consistency. We provide full traceability and Certificates of Analysis (COA) are available for all shipments, detailing key parameters such as assay (HPLC), chiral purity, and residual solvent content. Our quality commitment aligns with the standards expected for high-value biochemical research reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Assay (HPLC)	≥ 95.0%
Chiral Purity	≥ 99.0% (by chiral HPLC)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.