Description

3-Carboxy-2,2,5,5-Tetramethyl-3-Pyrrolin-1-Yloxy, Free Radical is a stable nitroxide radical compound, widely recognized as a spin label and spin trap in electron paramagnetic resonance (EPR) spectroscopy. Its significance lies in its ability to probe molecular dynamics, structure, and local environments in complex chemical and biological systems without significantly perturbing them. This makes it an essential tool for researchers and developers in advanced materials science, structural biology, and polymer chemistry who require precise, non-invasive analytical techniques.

Application

• Spin Labeling in Biophysics: Used to tag proteins, nucleic acids, and membranes for studying conformational changes, dynamics, and distances via site-directed spin labeling (SDSL) EPR.
• Polymer Science & Material Characterization: Acts as a probe for investigating polymer chain mobility, cross-linking density, phase separation, and the microstructure of advanced materials like hydrogels and composites.
• Antioxidant & Radical Scavenging Studies: Employed as a standard or probe to measure antioxidant capacity and to trap and identify short-lived reactive oxygen species (ROS) in chemical and biological systems.
• Structural Biology & Drug Discovery: Facilitates the determination of protein structures and protein-ligand interactions through techniques like double electron-electron resonance (DEER) or PELDOR.
• Oxidative Stress & Medical Research: Utilized in model systems to simulate and study oxidative damage mechanisms and the protective effects of novel therapeutic compounds.
• Quality Control in Chemical Synthesis: Serves as a reference standard or radical initiator in controlled synthetic processes and for calibrating EPR instrumentation.

Basic Information

Product Name	3-Carboxy-2,2,5,5-Tetramethyl-3-Pyrrolin-1-Yloxy, Free Radical
CAS No.	111930-19-9
Molecular Formula	C9H14NO3•
Molecular Weight	184.21 g/mol (radical form)
Synonyms	3-Carboxy-PROXYL; 3-Carboxy-2,2,5,5-tetramethylpyrrolidine-1-oxyl; 3-Carboxy-2,2,5,5-tetramethyl-1-pyrrolidinyloxy; 3-CP; Carboxy-PROXYL; 1-Oxyl-2,2,5,5-tetramethyl-3-carboxypyrroline; 3-Carboxy-2,2,5,5-tetramethyl-δ3-pyrrolin-1-oxyl; Free Radical Probe CTMCP
EINECS	Contact for details

Quality Control

Our 3-Carboxy-2,2,5,5-Tetramethyl-3-Pyrrolin-1-Yloxy, Free Radical is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and high purity, which is critical for sensitive EPR spectroscopy applications. Each lot undergoes comprehensive analytical testing, including EPR for radical purity and HPLC for chemical purity. A Certificate of Analysis (COA) detailing identity, purity, and assay is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C under an inert atmosphere (e.g., nitrogen or argon) to minimize oxidation and degradation. Keep away from strong oxidizing agents and reducing agents. For long-term stability, desiccate if the container is opened frequently.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Identification (EPR)	Characteristic triplet signal
Assay (HPLC)	≥ 98.0% (Chemical Purity)
Radical Purity (EPR)	≥ 95.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.