Description

Phenylalanyl-Prolyl-Arginine Nitrile is a synthetic tripeptide derivative of significant interest in advanced biochemical research and pharmaceutical development. Its core value lies in its role as a potent and selective inhibitor of serine proteases, particularly those involved in critical physiological pathways. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors who require high-purity biochemical tools for drug discovery, enzyme mechanism studies, and diagnostic applications.

Application

• Biochemical Research: As a key inhibitor for studying the activity and regulation of serine proteases like thrombin, trypsin, and plasmin.
• Pharmaceutical Development: Serves as a lead compound or intermediate in the design of novel therapeutics targeting coagulation disorders, inflammatory diseases, and cancer.
• Diagnostic Reagent Formulation: Used in the development of in-vitro diagnostic kits and assays for measuring specific protease activity in clinical samples.
• Enzyme Kinetics Studies: Provides a high-affinity ligand for detailed kinetic analysis and characterization of protease-substrate interactions.
• Cell Biology & Proteomics: Employed to selectively inhibit proteolytic activity in cell culture systems and complex biological samples to elucidate protein function.

Basic Information

Item	Detail
Product Name	Phenylalanyl-Prolyl-Arginine Nitrile
CAS No.	111009-86-0
Molecular Formula	C₂₀H₂₉N₇O₃
Molecular Weight	415.49 g/mol
Synonyms	Phe-Pro-Arg-CN; H-Phe-Pro-Arg-CN; Phenylalanine-Proline-Arginine Nitrile; Thrombin Inhibitor I; (2S)-5-[[(2S)-1-[(2S)-2-Amino-3-phenylpropanoyl]pyrrolidin-2-yl]carbonylamino]-2-cyanopentan-1-aminium; Boc-D-Phe-Pro-Arg-H (reduced form analog); Serine Protease Inhibitor (tripeptide nitrile)
EINECS	Contact for details

Quality Control

Our Phenylalanyl-Prolyl-Arginine Nitrile is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity determination and spectroscopic identification (NMR, MS). Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.