Description

Calpain Inhibitor I is a potent, cell-permeable, and irreversible inhibitor of the calcium-dependent cysteine proteases calpain I and II. This compound is a critical research tool for studying the role of calpain-mediated proteolysis in cellular processes such as apoptosis, signal transduction, and cytoskeletal remodeling. It is essential for researchers in pharmaceutical development, neuroscience, and cardiovascular disease research, providing a reliable means to elucidate pathological mechanisms and validate therapeutic targets.

Application

• Biomedical Research: Fundamental tool for investigating calpain's role in cellular signaling, necrosis, and apoptosis.
• Neurodegenerative Disease Studies: Used in models of Alzheimer's disease, Parkinson's disease, and traumatic brain injury to inhibit calpain-induced proteolysis.
• Cardiovascular Research: Applied in studies of myocardial ischemia-reperfusion injury and cardiac hypertrophy.
• Muscular Dystrophy Research: Employed to understand and potentially mitigate calpain-3 mediated muscle fiber degradation.
• Cancer Biology: Utilized to study the involvement of calpains in tumor cell migration, invasion, and metastasis.
• In Vitro Biochemical Assays: Serves as a key control and specificity agent in enzyme activity assays and high-throughput screening.

Basic Information

Product Name	Calpain Inhibitor I
CAS No.	110044-82-1
Molecular Formula	C₂₀H₃₈N₄O₄
Molecular Weight	398.54 g/mol
Synonyms	ALLN; N-Acetyl-Leu-Leu-Norleucinal; Ac-LLN-CHO; Calpain Inhibitor I, ALLN; MDL 28170; Z-LLN-CHO; N-Acetyl-Leu-Leu-Nle-al; (2S)-2-Acetamido-4-methyl-N-[(2S)-1-[[(2S)-1-oxo-1-[(2S)-2-[[(2S)-2-acetamido-4-methylpentanoyl]amino]pentyl]amino]pentan-2-yl]pentanamide
EINECS	Contact for details

Quality Control

Our Calpain Inhibitor I is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure identity, potency, and consistency. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided to support your research integrity.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. The product is hygroscopic (moisture-sensitive). For long-term storage, consider under an inert atmosphere. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Biological Activity (In vitro assay)	IC₅₀ ≤ 10 µM for Calpain I

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.