Description

Butoxycarbonylglutamyl-Lysyl-Lysine-4-Methylcoumarinyl-7-Amide is a specialized synthetic peptide derivative containing a fluorogenic coumarin moiety. This compound is primarily valued as a highly sensitive and specific substrate for proteolytic enzyme research, particularly for enzymes like cathepsins and other lysosomal proteases. It is an essential tool for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require precise enzyme activity assays and inhibitor screening.

Application

• Protease/Enzyme Activity Assays: Used as a fluorogenic substrate to measure the kinetic activity of specific proteases like cathepsins B, L, and H.
• Drug Discovery & High-Throughput Screening (HTS): Enables the identification and characterization of novel protease inhibitors for therapeutic development.
• Biochemical Research: A critical reagent for studying protease function, mechanism of action, and regulation in various biological pathways.
• Diagnostic Kit Development: Serves as a key component in developing sensitive in-vitro diagnostic tests for diseases involving protease dysregulation.
• Cell Biology & Immunology Studies: Used to probe protease activity in live cells, tissue extracts, and immune response mechanisms.
• Quality Control in Biologics Manufacturing: Employed to monitor and validate the presence or activity of specific enzymes during production processes.

Basic Information

Product Name	Butoxycarbonylglutamyl-Lysyl-Lysine-4-Methylcoumarinyl-7-Amide
CAS No.	106984-12-7
Molecular Formula	C₃₆H₅₆N₆O₁₀
Molecular Weight	740.87 g/mol
Synonyms	Boc-Glu-Lys-Lys-MCA; Boc-Glu-Lys-Lys-AMC; Z-Glu-Lys-Lys-MCA (common alternate); t-Butoxycarbonyl-L-glutamyl-L-lysyl-L-lysine 7-amido-4-methylcoumarin; Cathepsin Substrate; Fluorogenic Peptide Substrate; (7-(4-Methylcoumarinyl)amide)-Boc-Glu-Lys-Lys; Boc-EKK-AMC
EINECS	Contact for details

Quality Control

Our Butoxycarbonylglutamyl-Lysyl-Lysine-4-Methylcoumarinyl-7-Amide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing including HPLC for purity, mass spectrometry for identity confirmation, and functional activity validation. Certificates of Analysis (COA) with detailed chromatographic data and specifications are provided to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Validated with reference enzyme

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.