Description

Leupeptin is a potent, cell-permeable protease inhibitor that specifically targets serine, cysteine, and threonine proteases. This compound is a critical research tool for studying proteolytic pathways, preventing unwanted protein degradation in biological samples. It is essential for scientists and manufacturers in the fields of biochemical research, pharmaceutical development, and diagnostic kit production.

Application

• Biochemical Research: Used as a standard protease inhibitor in cell lysis buffers and protein extraction protocols to preserve protein integrity.
• Pharmaceutical R&D: Employed in drug discovery programs targeting proteases involved in diseases such as cancer, inflammation, and neurodegenerative disorders.
• Diagnostic Manufacturing: Incorporated into reagent formulations for diagnostic assays to stabilize enzymes and improve test shelf-life and accuracy.
• Enzymology Studies: Serves as a specific inhibitor to characterize and study the activity of proteases like trypsin, plasmin, and cathepsin B.
• Cell Culture & Tissue Engineering: Added to media to inhibit extracellular matrix degradation, aiding in the maintenance of primary cell cultures.
• Food Science Research: Investigated for its potential to control enzymatic browning and spoilage in food products by inhibiting specific proteases.

Basic Information

Product Name	Leupeptin
CAS No.	103476-89-7
Molecular Formula	C₂₀H₃₈N₆O₄
Molecular Weight	426.55 g/mol
Synonyms	Acetyl-Leu-Leu-Arg-al; N-Acetyl-L-leucyl-L-leucyl-L-arginal; Leupeptin Hemisulfate; Protease Inhibitor, Leupeptin; NSC 332500; Antibiotic, Leupeptin; Ac-Leu-Leu-Arg-H
EINECS	Contact for details

Quality Control

Our Leupeptin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the high standards required for research and industrial applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be kept in a dry environment. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 98% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.