Description

Ala-Ala-Phe-Chloromethylketone Tfa is a specialized tripeptide chloromethyl ketone derivative, a potent and irreversible inhibitor of serine proteases. This compound is critical for biochemical research and pharmaceutical development, enabling precise study and modulation of enzymatic activity. It is primarily utilized by researchers and manufacturers in the fields of protease inhibitor development, peptide chemistry, and drug discovery.

Application

• Protease Inhibition Studies: Acts as a specific, irreversible inhibitor for characterizing serine protease mechanisms and kinetics.
• Biochemical Research Reagent: Used in enzymatic assays to study substrate specificity and enzyme active sites.
• Pharmaceutical Intermediate: Serves as a key building block in the synthesis of more complex therapeutic protease inhibitors.
• Medicinal Chemistry: Employed in structure-activity relationship (SAR) studies to design novel drug candidates targeting proteases.
• Academic & Industrial R&D: Essential tool for universities, biotech, and pharmaceutical companies exploring protease-related pathways.

Basic Information

Product Name	Ala-Ala-Phe-Chloromethylketone Tfa
CAS No.	102129-66-8
Molecular Formula	C20H25ClF3N3O6
Molecular Weight	495.88 g/mol
Synonyms	Ala-Ala-Phe-CMK TFA; L-Alanyl-L-alanyl-L-phenylalanine chloromethyl ketone trifluoroacetate; AAPF-CMK; N-[(Benzyloxy)carbonyl]-L-alanyl-L-alanyl-L-phenylalanine chloromethyl ketone; Z-Ala-Ala-Phe-CH2Cl; Protease Inhibitor AAPF-CMK; Chloromethyl ketone inhibitor of chymotrypsin; Acetyl-Ala-Ala-Phe-chloromethylketone (Tfa salt)
EINECS	Contact for details

Quality Control

Our Ala-Ala-Phe-Chloromethylketone Tfa is manufactured under strict quality protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, freezer environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.