Description

Calyculin A is a potent and selective cell-permeable inhibitor of protein phosphatases 1 (PP1) and 2A (PP2A). This compound is a critical biochemical tool for researchers studying cellular signaling pathways, particularly those involving serine/threonine phosphorylation. It is primarily utilized in life science research, pharmaceutical development, and biochemical studies to induce hyperphosphorylation of cellular proteins and investigate tumor promotion mechanisms.

Application

• Biochemical Research: A standard tool for inhibiting PP1 and PP2A in cell-based assays and in vitro studies to elucidate phosphorylation-dependent signaling cascades.
• Cancer Research: Used to study tumor promotion, cell cycle regulation, and apoptosis by modulating key phosphatase activities.
• Neuroscience: Employed in research on synaptic plasticity, neurotransmitter release, and neuronal signaling pathways affected by phosphatase inhibition.
• Pharmaceutical Screening: Serves as a reference compound or positive control in high-throughput screens for novel phosphatase inhibitors or modulators.
• Cell Biology: Applied to induce actin cytoskeleton reorganization and morphological changes in cells for functional studies.
• Toxicology Studies: Used to investigate the mechanisms of natural marine toxins and their effects on cellular physiology.

Basic Information

Product Name	Calyculin A
CAS No.	101932-71-2
Molecular Formula	C₅₀H₈₁N₄O₁₅P
Molecular Weight	1009.17 g/mol
Synonyms	Calyculin A; Calyculin-A; Calyculin A from *Discodermia calyx*; (1S,2R,4R,7S,9S,11S,13S,14R,16S,18S,20S,22S,24S,25S,26R,27S,28S,30S,32S,33S,34R,35S,36S,37S,38S,39S,40S,41S,42S,43S,44S,45S,46S,47S,48S,49S,50S)-50-[(2S,3S,4S,5S,6R)-3,4-Dihydroxy-6-methyl-5-(methylamino)oxan-2-yl]oxy-2,4,7,9,11,13,14,16,18,20,22,24,25,26,27,28,30,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49-pentacontahydroxy-1,49-dimethyl-15,31-dioxo-12λ⁵-1H,15H,31H-12,13,15,31-tetraoxa-12λ⁵-phospha-2-azatricyclo[28.12.1.1¹⁴,¹⁸]pentaconta-3,5,7,9,19,21,23,25,27,29,37,39,41,43,45-pentadecaene-12-sulfide
EINECS	Contact for details

Quality Control

Our Calyculin A is produced and handled under strict quality protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot is thoroughly characterized using advanced analytical techniques, including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your research integrity and documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, ethanol, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.