Description

25-Hydroxyvitamin D3 3-(n-(4-Azido-2-Nitrophenyl)Glycinate) is a chemically modified derivative of 25-Hydroxyvitamin D3, a key metabolite in the vitamin D pathway, designed for advanced biochemical research and pharmaceutical development. This compound matters as a versatile photoaffinity labeling reagent, enabling the study of vitamin D receptor interactions and metabolic pathways through covalent cross-linking. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors for developing targeted therapies and diagnostic tools.

Application

• Photoaffinity labeling studies for mapping vitamin D receptor (VDR) binding sites and protein interactions.
• Biochemical research tool for investigating the metabolism and biological activity of vitamin D analogs.
• Development of novel pharmaceutical agents targeting vitamin D-related pathways, such as calcium homeostasis and immune modulation.
• Probe synthesis for activity-based protein profiling (ABPP) in proteomics research.
• Intermediate in the synthesis of specialized vitamin D derivatives for clinical and diagnostic applications.
• Use in molecular biology for studying gene regulation mediated by the vitamin D endocrine system.

Basic Information

Product Name	25-Hydroxyvitamin D3 3-(n-(4-Azido-2-Nitrophenyl)Glycinate)
CAS No.	101396-04-7
Molecular Formula	C33H45N5O6
Molecular Weight	607.75 g/mol
Synonyms	25-Hydroxyvitamin D3 3-(N-(4-Azido-2-nitrophenyl)glycinate); 25-Hydroxycholecalciferol 3-(4-Azido-2-nitrophenylglycinate); Calcifediol 3-(ANP-Glycinate); 25(OH)D3-ANP-Gly; Vitamin D3 Metabolite Photoaffinity Probe; 25-Hydroxyvitamin D3 ANP Derivative; 25-Hydroxyvitamin D3 Photoaffinity Label; 3-(N-(4-Azido-2-nitrophenyl)glycinyl)-25-hydroxycholecalciferol
EINECS	Contact for details

Quality Control

Our 25-Hydroxyvitamin D3 3-(n-(4-Azido-2-Nitrophenyl)Glycinate) is produced under stringent quality management systems to ensure high purity and batch-to-batch consistency suitable for research and development. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. Due to its hygroscopic and light-sensitive nature, the material should be handled in a controlled, dry environment and allowed to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Conforms
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.