Description

Phorbol 12-Myristate 13-Acetate (CAS NO 16561-29-8) is a potent and specific protein kinase C (PKC) activator widely used as a critical biochemical tool in life science research. Its primary value lies in its ability to reliably stimulate PKC-dependent signaling pathways, making it indispensable for studying cellular processes like proliferation, differentiation, and apoptosis. This compound is essential for researchers and manufacturers in the fields of immunology, oncology, and pharmacology, where it is used to model disease states, screen drug candidates, and elucidate complex signal transduction mechanisms.

Application

• Biomedical Research: A key reagent for activating protein kinase C (PKC) in cell culture studies to investigate signal transduction, tumor promotion, and immune cell activation.
• Immunology Studies: Used to stimulate T-cell proliferation, neutrophil oxidative burst, and cytokine production in experimental models of inflammation and immune response.
• Cancer Research: Employed as a tumor promoter in carcinogenesis studies and to probe PKC's role in cancer cell survival, proliferation, and drug resistance mechanisms.
• Drug Discovery & Screening: Serves as a positive control and challenge agent in high-throughput screens for novel PKC inhibitors and anti-inflammatory compounds.
• Cell Signaling Pathways: A fundamental tool for dissecting the downstream effects of PKC activation on transcription factors, enzyme activity, and gene expression.
• Dermatological Research: Applied in skin models to study hyperproliferative and inflammatory conditions linked to PKC activity.

Basic Information

Product Name	Phorbol 12-Myristate 13-Acetate
CAS No.	16561-29-8
Molecular Formula	C36H56O8
Molecular Weight	616.83 g/mol
Synonyms	PMA; TPA; 12-O-Tetradecanoylphorbol-13-acetate; Phorbol 12-Tetradecanoate 13-Acetate; NSC 350836; 4α-Phorbol 12-myristate 13-acetate; Tetradecanoylphorbol Acetate; Forbol 12-Miristato 13-Acetato
EINECS	Contact for details

Quality Control

Our Phorbol 12-Myristate 13-Acetate is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including identity confirmation by spectroscopic methods and purity assessment by HPLC. A Certificate of Analysis (COA) detailing specific results for purity, related substances, and other critical parameters is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. Due to its sensitivity to light and oxidation, prolonged exposure to ambient conditions should be avoided. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Solubility	Soluble in DMSO, ethanol, acetone; insoluble in water
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.