Description

n-ε-2,4-Dnp-L-Lysine Hydrochloride is a chemically modified derivative of the essential amino acid L-lysine, specifically functionalized with a 2,4-dinitrophenyl (DNP) group at the ε-amino position. This compound serves as a critical reference standard and analytical reagent for the detection and quantification of lysine residues in proteins and peptides. It is primarily utilized by research institutions, pharmaceutical R&D laboratories, and quality control facilities involved in protein chemistry, biochemical analysis, and method development.

Application

• Biochemical Research: Used as a key reference standard in the study of protein modification, amino acid analysis, and enzyme kinetics.
• Analytical Chemistry: Serves as a calibration standard for chromatographic methods (HPLC, LC-MS) aimed at detecting DNP-labeled amino acids.
• Pharmaceutical Development: Employed in the characterization of biopharmaceuticals and in assays to monitor lysine-specific conjugation or modification reactions.
• Diagnostic Reagent Manufacturing: Acts as a critical component in the production of test kits and reagents for clinical and research diagnostics related to amino acid metabolism.
• Food & Nutrition Science: Applied in advanced methods for the accurate determination of available lysine content in protein-rich food and feed samples.
• Method Validation: Essential for validating analytical procedures in compliance with Good Laboratory Practice (GLP) and other regulatory guidelines.

Basic Information

Product Name	n-ε-2,4-Dnp-L-Lysine Hydrochloride
CAS No.	14455-27-7
Molecular Formula	C₁₂H₁₇N₅O₆ • HCl
Molecular Weight	363.75 g/mol
Synonyms	Nε-(2,4-Dinitrophenyl)-L-lysine hydrochloride; L-Lysine, N6-(2,4-dinitrophenyl)-, hydrochloride; Nε-DNP-L-lysine hydrochloride; 2,4-DNP-lysine HCl; (S)-2-Amino-6-((2,4-dinitrophenyl)amino)hexanoic acid hydrochloride; DNP-Lysine hydrochloride; ε-DNP-L-lysine HCl; Lys(DNP) hydrochloride
EINECS	Contact for details

Quality Control

Our n-ε-2,4-Dnp-L-Lysine Hydrochloride is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification, and specific optical rotation verification, to ensure it meets stringent specifications for use as a high-grade analytical standard. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, batch-to-batch consistency, and compliance with research and quality control requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.