Description

α-Amanitin is a highly potent bicyclic octapeptide and a principal toxic component of the *Amanita phalloides* mushroom, known for its specific inhibition of RNA polymerase II. This compound is of critical importance in biochemical research for studying eukaryotic transcription mechanisms and apoptosis pathways. It is primarily utilized by researchers in pharmaceutical development, toxicology, and molecular biology for its role as a precise biochemical tool.

Application

• Biochemical Research: Used as a selective inhibitor of RNA polymerase II to study gene transcription, mRNA synthesis, and nuclear processes.
• Toxicology Studies: Employed in research on mushroom poisoning (amatoxin poisoning) mechanisms, liver toxicity (hepatotoxicity), and the development of potential antidotes.
• Cell Biology & Apoptosis Research: Applied to induce programmed cell death in experimental models to investigate apoptotic pathways and cell cycle arrest.
• Pharmaceutical Development: Serves as a reference standard and tool compound in drug discovery, particularly for projects targeting transcription or designing toxin-binding therapies.
• Diagnostic Development: Used in the development and calibration of analytical methods (e.g., HPLC-MS, ELISA) for the detection of amatoxins in biological and environmental samples.

Basic Information

Product Name	α-Amanitin
CAS No.	11030-71-0
Molecular Formula	C39H54N10O14S
Molecular Weight	918.97 g/mol
Synonyms	α-Amanitin; α-Amanitine; Amanitin alpha; (3S,6S,9S,12R,15S,18S,21S,24S,30S,33S)-30-[(2S)-2-Amino-2-carboxyethyl]-33-(3-hydroxy-2-methylpropanoyl)-21-inosyl-12-(4-methoxy-1H-indol-3-yl)-15-methyl-3,6,9,24-tetrakis(oxidanyl)-1,4,7,10,13,16,19,22,25,28,31-undecaoxo-1λ6,4λ6,7λ6,10λ6,13λ6,16λ6,19λ6,22λ6,25λ6,28λ6,31λ6-dodecathia-34-azatetracyclo[31.2.2.214,17.220,23]henpentacontane-2,5,8,11,14,17,20,23,26,29,32,34-dodecone; Amatoxin
EINECS	Contact for details

Quality Control

Our α-Amanitin is produced and handled under strict quality protocols suitable for research applications. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure consistency and reliability. A detailed Certificate of Analysis (COA) with batch-specific data is provided to support your research integrity and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to equilibrate to room temperature before opening to prevent condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water, methanol, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.